Набор для флуорометрического анализа поглощения глюкозы / Glucose Uptake Fluorometric Assay Kit
- Detection instrument: Fluorescence microplate reader (Ex/Em=530 nm/590 nm)
- Detection method: Fluorescence method
Manual
Detection principle
2-DG is up-taken by the cells, converted to 2-DG-6P, which is catalyzed by glucose dehydrogenase to produce 6PDG. Meanwhile, NADP+ is converted to NADPH. The generated NADPH converts the probe into fluorescent substances under the action of myocardial yellow transferase. The glucose uptake can be calculated by measuring the fluorescence intensity at the excitation wavelength of 530 nm and the emission wavelength of 590 nm.
Performance characteristics
| Sample type |
cells |
| Sensitivity |
0.02 nmol/μL |
| Detection range |
0.02-0.3 nmol/μL |
| Detection method |
Fluorescence method |
| Assay type |
Quantitative |
| Assay time |
100 min |
| Other instruments required |
Micropipettor, Incubator, Water bath |
| Other reagents required |
KRPH solution (20 mM Hepes, 5 mM KH2PO4, 1 mM MgSO4, 1 mM CaCl2, 136 mM NaCl, 4.7 mM KCl, 2% BSA, pH 7.4) |
| Storage |
-20℃ |
| Valid period |
12 months |
Информация для заказа| Область использования: | Производство: | Elabscience | | Метод: | | | Объем: | 40 тестов | | Кат. номер: | E-BC-F041 | | Цена (с НДС 20%): | по запросу | В корзину  | Наименование: Набор для флуорометрического анализа поглощения глюкозы / Glucose Uptake Fluorometric Assay Kit.
Примечание: |
|
