- Detection method: Colorimetric method
Manual
Detection principle
Glucose-6-phosphate (G6P) is a molecule generated by phosphorylation of hydroxyl groups on the sixth carbon of glucose under the catalysis of hexokinase. It is a common small molecule of sugar metabolism in cells and participates in biochemical pathways such as glycolysis and pentose phosphate pathway. In the first reaction of glycolysis, glucose is catalyzed by hexokinase to produce glucose-6-phosphate, which is then catalyzed by phosphoglucose isomerase to form fructose-6-phosphate to continue the other steps of glycolysis: In the pentose phosphate pathway, glucose-6-phosphate is the first substrate, and this process is also the main way to generate NADPH. In addition to these two metabolic pathways, glucose-6-phosphate can also be converted into glycogen or starch and stored.
Performance characteristics
| Synonyms |
G6P |
| Sample type |
Serum, plasma, animal tissue |
| Sensitivity |
5.6 μmol /L |
| Detection range |
5.6-500 μmol/L |
| Detection method |
Colorimetric method |
| Assay type |
Quantitative |
| Assay time |
35 min |
| Precision |
Average inter-assay CV: 4.300%Average intra-assay CV: 2.100% |
| Other instruments required |
Pipettor, Water bath, Centrifuge |
| Storage |
-20℃ |
| Valid period |
12 months |
Dilution of sample
It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (5.6-500 μmol/L).
The recommended dilution factor for different samples is as follows (for reference only):
|
Sample type
|
Dilution factor
|
|
Human plasma
|
1
|
|
Mouse serum
|
2-3
|
|
Rat plasma
|
1
|
|
Porcine serum
|
1
|
|
10% Rat spleen tissue homogenate
|
2-3
|
|
10% Rat heart tissue homogenate
|
2-3
|
|
10% Rat liver tissue homogenate
|
2-3
|
|
10% Mouse lung tissue homogenate
|
1
|
Note: The diluent is reagent 1.
