- Detection method: Colorimetric method
Manual
Detection principle
Under the presence of G6PDH, glucose-6-phosphoric acid is oxidized to 6-PG. In this reaction, NADP+ is reduced to NADPH. Under the action of electron coupling reagent 1-MPMS, NADPH reducts wST-8 to form orange formazan, which has the maximum absorption peak at about 450 nm. Formazan generated in the reaction system is proportional to the activity of G6DPH in the sample.
Performance characteristics
| Synonyms |
G-6-PD, G6PDH |
| Sample type |
Serum, plasma, animal tissue |
| Sensitivity |
0.01 U/L |
| Detection range |
0.01-50 U/L |
| Detection method |
Colorimetric method |
| Assay type |
Enzyme Activity |
| Assay time |
20 min |
| Precision |
Average inter-assay CV: 5.800%Average intra-assay CV: 2.100% |
| Other instruments required |
Pipettor, Water bath, Centrifuge |
| Storage |
-20℃ |
| Valid period |
12 months |
Dilution of sample
It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (0.01-50 U/L).
The recommended dilution factor for different samples is as follows (for reference only):
|
Sample type
|
Dilution factor
|
|
Human serum
|
1
|
|
Rat serum
|
1
|
|
Rabbit serum
|
1
|
|
Cynomolgus monkey
|
1
|
|
10% Mouse liver tissue homogenate
|
3-5
|
|
10% Rat kidney tissue homogenate
|
1
|
|
10% Rat spleen tissue homogenate
|
5-10
|
|
10% Mouse brain tissue homogenate
|
1
|
Note: The diluent is reagent 1.
