Performance characteristics
| Synonyms |
FFA/NEFA |
| Sample type |
Serum, plasma, animal tissue, cells |
| Sensitivity |
0.58 μmol/L |
| Detection range |
0.58-20 μmol/L |
| Detection method |
Fluorescence method |
| Assay type |
Quantitative |
| Assay time |
70 min |
| Precision |
Average inter-assay CV: 5.300%Average intra-assay CV: 4.200% |
| Other instruments required |
Micropipette, Vortex mixer, Centrifuge |
| Storage |
-20℃ |
| Valid period |
12 months |
Dilution of sample
It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (0.58-20 μmol/L).
The recommended dilution factor for different samples is as follows (for reference only):
|
Sample type
|
Dilution factor
|
|
Human serum
|
10-20
|
|
Rat serum
|
200-400
|
|
Mouse plasma
|
100-300
|
|
Rabbit serum
|
100-300
|
|
10% Rat liver tissue homogenate
|
200-400
|
|
10% Mouse kidney tissue homogenate
|
200-400
|
|
10% Rat brain tissue homogenate
|
200-400
|
|
10% Rat lung tissue homogenate
|
200-400
|
Note: The diluent is reagent 1.
