Набор для колориметрического анализа неэтерифицированных свободных жирных кислот (NEFA) / Non-esterified Free Fatty Acids (NEFA) Colorimetric Assay Kit
- Detection instrument: Semi-automatic biochemical analyzer (546 nm), automatic biochemical analyzer (546 nm), Microplate reader
- Detection method: Colorimetric method
Manual
Detection principle
NEFA and can react with coenzyme A and form acetyl-CoA under the catalysis of acetyl-CoA-synthetase (ACS). Acetyl-CoA can produce H2O2 when catalyzed by acetyl-CoA-oxidase (ACOD). Then H2O2 react with TOOS and 4-amino-antipyrine (4-APP) to generate a colored substrate under the catalysis of peroxidase (POD). The colored substrate has a maximum absorption peak at 546 nm. Measure the OD value at 546 nm and calculate the NEFA content indirectly.
Performance characteristics
Synonyms |
NEFA |
Sample type |
Serum,plasma,tissue homogenate,cells,cell supernatant |
Detection range |
0.01-3.0 mmol/L |
Detection method |
Colorimetric method |
Assay type |
Quantitative |
Other reagents required |
Normal saline (0.9% NaCl) |
Storage |
2-8℃ |
Valid period |
12 months |
Информация для заказаОбласть использования: | Производство: | Elabscience | Метод: | | Объем: | 92 теста | Кат. номер: | E-BC-K014 | Цена (с НДС 20%): | по запросу | В корзину ![](/catalog/cart.gif) | Наименование: Набор для колориметрического анализа неэтерифицированных свободных жирных кислот (NEFA) / Non-esterified Free Fatty Acids (NEFA) Colorimetric Assay Kit. Примечание: |
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