- Detection method: Colorimetric method
Manual
Detection principle
Under the condition of weak acidity, non-esterified free fatty acids (NEFA) react with nantokite to form copper soap, which has a specific absorption peak at 715nm. The content of NEFA can be calculated by measuring the OD value at 715 nm.
Performance characteristics
| Synonyms |
NEFA |
| Sample type |
Serum,animal tissue |
| Sensitivity |
0.05 mmol/L |
| Detection range |
0.05-2.0 mmol/L |
| Detection method |
Colorimetric method |
| Assay type |
Quantitative |
| Assay time |
60 min |
| Precision |
Average inter-assay CV: 6.100%Average intra-assay CV: 2.200% |
| Other instruments required |
Vortex mixer, Micropipettor |
| Storage |
2-8℃ |
| Valid period |
12 months |
Dilution of sample
It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (0.05-2.0 mmol/L).
The recommended dilution factor for different samples is as follows (for reference only):
|
Sample type
|
Dilution factor
|
|
Rat liver tissue homogenate
|
1-3
|
|
Rat heart tissue homogenate
|
1
|
|
Rat kidney tissue homogenate
|
1
|
|
Mouse liver tissue homogenate
|
1-3
|
|
Rat serum
|
1
|
|
Mouse serum
|
1
|
|
Human serum
|
1
|
Note: The diluent is Reagent 1.
