- Detection method: Colorimetric method
Manual
Detection principle
N-[3-(2-furyl)acryloyl]-L-phenylalanylglycylglycine (FAPGG) have the maximum absorption peak at 340 nm, angiotensin converting enzyme catalyze N-(3[2-Furyl ]Acryloyl)-Phe-Gly-Gly to produce FAP and GG, and the absorbance at 340 nm will be decreased. The activity of ACE1 can be calculated indirectly by measuring the decrease in absorbance at 340 nm.
Performance characteristics
| Synonyms |
ACE1 |
| Sample type |
Serum, plasma, animal tissue |
| Sensitivity |
27.5 U/L |
| Detection range |
27.5- 682 U/L |
| Detection method |
Colorimetric method |
| Assay type |
Enzyme Activity |
| Assay time |
10-90 min |
| Precision |
Average inter-assay CV: 4.400%Average intra-assay CV: 3% |
| Other instruments required |
Micropipettor, Vortex mixer, Incubator, Centrifuge |
| Other reagents required |
PBS (0.01 M, pH 7.4) |
| Storage |
2-8℃ |
| Valid period |
12 months |
Dilution of sample
It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (27.5- 682 U/L).
The recommended dilution factor for different samples is as follows (for reference only):
|
Sample type
|
Dilution factor
|
|
Human serum
|
1
|
|
Mouse serum
|
1
|
|
Rat plasma
|
1
|
|
10% Rat liver tissue homogenate
|
1
|
|
10% Rat kidney tissue homogenate
|
1
|
|
10% Rat lung tissue homogenate
|
3-5
|
Note: The diluent is normal saline.
