Arachidonic acid generates prostaglandins under the catalysis of cyclooxygenase, and under the action of cofactor (heme), cyclooxygenase activates peroxidase activity, converting fluorescent red into fluorescent substance.
Performance characteristics
Sample type
animal tissue
Sensitivity
0.03 U/L
Detection range
0.03-9.66 U/L
Detection method
Fluorescence method
Assay type
Enzyme Activity
Assay time
70 min
Precision
Average inter-assay CV: 5.0-7.0%Average intra-assay CV: 3.5-5.0%