H2S can form methylene blue in the presence of Fe 3+ and chromogenic agent.Methylene blue has a maximum absorption peak at 680 nm. H 2 S content can becalculated indirectly by measuring the OD value at 680 nm.
Performance characteristics
Sample type
serum;plasma;animal tissue
Sensitivity
1.97 μmol/L
Detection range
1.97-100 μmol/L
Detection method
Colorimetric method
Assay type
Quantitative
Assay time
60 min
Precision
Average inter-assay CV: 4.5-7.7%Average intra-assay CV: 2.6-4.4%