Набор для анализа активности лактатдегидрогеназы / Lactate Dehydrogenase (LDH) Activity Assay Kit
- Detection instrument: Microplate reader(440-460 nm,optimum wavelength: 450 nm)
- Detection method: Colorimetric method
Manual
Detection principle
Using coenzyme I as a hydrogen carrier, LDH catalyze lactic acid to produce pyruvate. Pyruvate reacted with 2, 4-dinitrophenylhydrazine to form pyruvate dinitrophenylhydrazone, which was red-brown in alkaline solution, and the color depth was proportional to pyruvate concentration. The activity of LDH could be calculated by measuring OD value.
Performance characteristics
| Synonyms |
LDH |
| Sample type |
Serum,plasma,hydrothorax,tissue,cells |
| Sensitivity |
6 U/L |
| Detection range |
6-1000 U/L |
| Detection method |
Colorimetric method |
| Assay type |
Enzyme Activity |
| Assay time |
45 min |
| Precision |
Average inter-assay CV: 2.400%Average intra-assay CV: 1.800% |
| Other instruments required |
Micropipettor, Multichannel pipette, Incubator |
| Other reagents required |
Normal saline (0.9% NaCl), PBS (0.01 M, pH 7.4) |
| Storage |
2-8℃ |
| Valid period |
12 months |
Информация для заказа| Область использования: | Производство: | Elabscience | | Метод: | | | Объем: | 40 тестов | | Кат. номер: | E-BC-K046-M | | Цена (с НДС 20%): | по запросу | В корзину  | Наименование: Набор для анализа активности лактатдегидрогеназы / Lactate Dehydrogenase (LDH) Activity Assay Kit.
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