- Detection method: Colorimetric method
Manual
Detection principle
Maltase catalyze the corresponding substrate to produce monosaccharide. Monosaccharide produce hydrogen peroxide under the action of oxidase. Hydrogen peroxide react with chromogenic agent to form red product. The activity of maltase can be calculated by detection the optical density with spectrophotometer at 505 nm.
Performance characteristics
| Sample type |
Animal tissue |
| Sensitivity |
6.32 U/mL |
| Detection range |
6.32-750 U/mL |
| Detection method |
Colorimetric method |
| Assay type |
Enzyme Activity |
| Assay time |
60 min |
| Precision |
Average inter-assay CV: 5.700%Average intra-assay CV: 2.700% |
| Other instruments required |
Micropipettor, Vortex mixer, Centrifuge, Water bath, Incubator |
| Other reagents required |
Normal saline (0.9% NaCl), PBS (0.01 M, pH 7.4) |
| Storage |
2-8℃ |
| Valid period |
12 months |
Dilution of sample
It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (6.32-750 U/mL).
The recommended dilution factor for different samples is as follows (for reference only):
|
Sample type
|
Dilution factor
|
|
10% Rat heart tissue homogenate
|
1
|
|
10% Rat liver tissue homogenate
|
1
|
|
10% Rat spleen tissue homogenate
|
1
|
|
10% Mouse intestinal tissue homogenate
|
1
|
|
10% Mouse kidney tissue homogenate
|
1
|
|
10% Mouse brain tissue homogenate
|
1
|
Note: The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4).
