Performance characteristics
| Synonyms |
LDH |
| Sample type |
Serum, plasma, animal tissue, hydrothorax, cells |
| Sensitivity |
0.11 U/L |
| Detection range |
0.11-39.9 U/L |
| Detection method |
Colorimetric method |
| Assay type |
Enzyme Activity |
| Assay time |
30 min |
| Precision |
Average inter-assay CV: 2.300%Average intra-assay CV: 2.300% |
| Other instruments required |
Microplate, Micropipettor, Vortex mixer, Water bath |
| Storage |
-20℃ |
| Valid period |
12 months |
Dilution of sample
It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (0.11-39.9 U/L).
The recommended dilution factor for different samples is as follows (for reference only):
|
Sample type
|
Dilution factor
|
|
Human serum
|
10-20
|
|
Dog serum
|
10-20
|
|
Mouse serum
|
50-100
|
|
Cynomolgus monkey serum
|
10-20
|
|
10% Rat spleen tissue homogenate
|
150-250
|
|
10% Rat liver tissue homogenate
|
250-350
|
|
10% Rat kidney tissue homogenate
|
250-350
|
|
10% Rat lung tissue homogenate
|
250-350
|
Note: The diluent is reagent 1.
