- Detection method: Fluorescence method
Manual
Detection principle
Glycogen produces glucose under the action of starch glycosidase, and glucose is catalyzed by glucose oxidase to produce hydrogen peroxide. In the presence of the peroxidase, hydrogen peroxide be oxidized to produce the fluorescence substrate. The fluorescence intensity at the excitation wavelength of 535 nm and emission wavelength of 587 nm is proportional to the glycogen content.
Performance characteristics
| Sample type |
Animal liver and muscle tissue |
| Sensitivity |
0.06 μg/mL |
| Detection range |
0.06-4.0 μg/mL |
| Detection method |
Fluorescence method |
| Assay type |
Quantitative |
| Assay time |
50min |
| Precision |
Average inter-assay CV: 6.600%Average intra-assay CV: 3.400% |
| Other instruments required |
Micropipettor, Incubator, Centrifuge |
| Storage |
-20℃ |
| Valid period |
12 months |
Dilution of sample
It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (0.06-4.0 μg/mL).
The recommended dilution factor for different samples is as follows (for reference only):
|
Sample type
|
Dilution factor
|
|
10% Rat liver tissue homogenate
|
3000-5000
|
|
10% Mouse muscle tissue homogenate
|
10-20
|
Note: The diluent is reagent 1. Liver samples can be diluted step by step.
