- Detection method: Fluorescence method
Manual
Detection principle
ALT catalyze the amino conversion reaction between alanine and α-ketoglutaric acid to produce pyruvic acid and glutamic acid. Under the action of pyruvate oxidase, pyruvic acid generates H2O2, which reacts with the non-fluorescent substance to form fluorescent substance under the action of peroxidase. The activity of ALT can be calculated by measuring the increase of fluorescence value at the excitation wavelength of 535 nm and the emission wavelength of 590 nm.
Performance characteristics
| Synonyms |
ALT |
| Sample type |
Serum, plasma, animal tissue |
| Sensitivity |
0.01 U/L |
| Detection range |
0.01-0.83 U/L |
| Detection method |
Fluorescence method |
| Assay type |
Enzyme Activity |
| Assay time |
75 min |
| Precision |
Average inter-assay CV: 9.800%Average intra-assay CV: 2.300% |
| Other instruments required |
Pipettor, Vortex mixer, Centrifuge |
| Storage |
-20℃ |
| Valid period |
12 months |
Dilution of sample
It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (0.01-0.83 U/L).
The recommended dilution factor for different samples is as follows (for reference only):
|
Sample type
|
Dilution factor
|
|
Human serum
|
10-15
|
|
Dog serum
|
5-10
|
|
Rat serum
|
10-15
|
|
10% Mouse heart tissue homogenate
|
100-120
|
|
10% Rat spleen tissue homogenate
|
10-15
|
|
10% Rat liver tissue homogenate
|
300-500
|
|
10% Rat kidney tissue homogenate
|
100-120
|
|
10% Rat lung tissue homogenate
|
100-120
|
Note: The diluent is reagent 1.
