- Detection method: Colorimetric method
Manual
Detection principle
Alkaline phosphatase (ALP) is a group of cytomembrane-related enzymes with hydrolysis and transfer activity, acting on a variety of phosphate substrates. ALP is a homologous dimerase and each catalytic site contains three metal ions. There are four isozymes in humans: tissue nonspecific ALP, intestinal ALP, placental ALP and genital cell ALP.
Performance characteristics
| Synonyms |
ALP |
| Sample type |
Serum, plasma, animal tissue, cells |
| Sensitivity |
0.27 U/L |
| Detection range |
0.27-50.8 U/L |
| Detection method |
Colorimetric method |
| Assay type |
Enzyme Activity |
| Assay time |
30 min |
| Precision |
Average inter-assay CV: 4.600%Average intra-assay CV: 1.200% |
| Other instruments required |
Micropipettor, Water bath, Incubator |
| Other reagents required |
Normal saline (0.9% NaCl) |
| Storage |
-20℃ |
| Valid period |
12 months |
Dilution of sample
It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (0.27-50.8 U/L).
The recommended dilution factor for different samples is as follows (for reference only):
|
Sample type
|
Dilution factor
|
|
Human serum
|
10-20
|
|
Mouse serum
|
20-30
|
|
Rat serum
|
10-20
|
|
Mouse plasma
|
10-20
|
|
HepG2 supernatant
|
8-12
|
|
10% Rat kidney tissue homogenate
|
400-1200
|
|
10% Mouse liver tissue homogenate
|
10-15
|
|
10% Rat lung tissue homogenate
|
50-100
|
|
10% Mouse brain tissue homogenate
|
30-50
|
|
Human urine
|
1
|
Note: The diluent is reagent 1.
