- Detection method: Colorimetric method
Manual
Detection principle
Urea can be decomposed into ammonia ion and carbon dioxide by urease. Ammonia ion can react with phenol chromogenic agent and form a blue substance in alkaline medium, and the production of the blue substance is proportional to the urea content which can be calculated with the colorimetric assay at 580 nm.
Performance characteristics
| Synonyms |
BUN |
| Sample type |
Serum,plasma,urine,saliva,milk |
| Sensitivity |
0.114 mmol/L |
| Detection range |
0.114-30 mmol/L |
| Detection method |
Colorimetric method |
| Assay type |
Quantitative |
| Assay time |
60 min |
| Precision |
Average inter-assay CV: 4.700%Average intra-assay CV: 4.600% |
| Other instruments required |
Micropipettor, Vortex mixer, Incubator |
| Other reagents required |
Normal saline (0.9% NaCl), PBS (0.01 M, pH 7.4) |
| Storage |
2-8℃ |
| Valid period |
12 months |
Dilution of sample
It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (0.114-30 mmol/L).
The recommended dilution factor for different samples is as follows (for reference only):
|
Sample type
|
Dilution factor
|
|
Rabbit plasma
|
1
|
|
Rat serum
|
1
|
|
Rat plasma
|
1
|
|
Human serum
|
1
|
|
Human saliva
|
1
|
|
Human milk
|
1
|
|
Human urine
|
30-60
|
|
Mouse urine
|
30-60
|
Note: The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4).
