In strong acidic and heating condition, urea can react with diacetyl to form red diazine compound. The depth of color is proportional to the content of urea. Because the instability of the diacetyl, the diacetyl oxime usually react with the strong acid firstly in the reaction system to generate diacetyl, then react with urea to generate the red diazine compound. The reaction equation is as follows:
Performance characteristics
| Synonyms |
BUN |
| Sample type |
Serum,plasma,saliva,urine |
| Sensitivity |
0.12 mmol/L |
| Detection range |
0.12-15 mmol/L |
| Detection method |
Colorimetric method |
| Assay type |
Quantitative |
| Assay time |
60 min |
| Precision |
Average inter-assay CV: 9.900%Average intra-assay CV: 4.900% |
| Other instruments required |
Vortex mixer, Micropipettor, Incubator， Water bath |
| Other reagents required |
Normal saline (0.9% NaCl), PBS (0.01 M, pH 7.4) |
| Storage |
2-8℃ |
| Valid period |
12 months |
Dilution of sample
It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (0.12-15 mmol/L).
The recommended dilution factor for different samples is as follows (for reference only):
|
Sample type
|
Dilution factor
|
|
Human urine
|
45-60
|
|
Human plasma
|
1
|
|
Human serum
|
1
|
|
Human saliva
|
1
|
|
Mouse serum
|
1
|
|
Rat urine
|
45-60
|
|
Rabbit plasma
|
1
|
Note: The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4).
