Detection principle
Uric acid can be used as an antioxidant to remove peroxide, hydroxyl and oxygen free radicals, chelate and transfer metal ions, protect vascular endothelial cells from damage. Uric Acid in protein-free filtrate reduce phosphotungstic acid to form tungsten blue, allantoin and carbon dioxide, the depth of blue color is proportional to the concentration of uric acid.
Performance characteristics
| Synonyms |
UA |
| Sample type |
Serum,plasma,urine |
| Sensitivity |
0.58 mg/L |
| Detection range |
0.58-100 mg/L |
| Detection method |
Colorimetric method |
| Assay type |
Quantitative |
| Assay time |
60 min |
| Precision |
Average inter-assay CV: 2.600%Average intra-assay CV: 1.800% |
| Other instruments required |
Vortex mixer, Micropipettor, Centrifuge |
| Other reagents required |
Normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4) |
| Storage |
2-8℃ |
| Valid period |
12 months |
Dilution of sample
It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (0.58-100 mg/L).
The recommended dilution factor for different samples is as follows (for reference only):
|
Sample type
|
Dilution factor
|
|
Human urine
|
8-10
|
|
Human serum
|
1
|
|
Dog serum
|
1
|
|
Rat serum
|
1
|
|
Mouse serum
|
1-2
|
|
Porcine serum
|
1
|
Note: The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4).
