Набор для колориметрического анализа гидроксипролина (HYP) / Hydroxyproline (HYP) Colorimetric Assay Kit
- Detection instrument: Spectrophotometer (550-570 nm, optimum wavelength: 558 nm)
- Detection method: Colorimetric method
Manual
Detection principle
The sample is hydrolyzed to generate free HYP, and hydroxyproline can produce oxidation product under the action of oxidizing agent. The generated oxidation product can react with chromogenic agent to produce burgundy. The concentration of hydroxyproline can be calculated by measuring the OD value at 558 nm.
Performance characteristics
| Synonyms |
HYP |
| Sample type |
serum, animal tissue and urine samples |
| Sensitivity |
0.032 μg/mL |
| Detection range |
0.032-10 μg/mL |
| Detection method |
Colorimetric method |
| Assay type |
Quantitative |
| Assay time |
45 min |
| Precision |
Average inter-assay CV: 5.700%Average intra-assay CV: 4.900% |
| Other instruments required |
Vortex mixer, Centrifuge, Water bath |
| Other reagents required |
6 mol/L Hydrochloric acid, Concentrated hydrochloric acid (12 mol/L), N-propyl alcohol |
| Storage |
2-8℃ |
| Valid period |
12 months |
Информация для заказа| Область использования: | Производство: | Elabscience | | Метод: | | | Объем: | 86 тестов | | Кат. номер: | E-BC-K062-S | | Цена (с НДС 20%): | по запросу | В корзину  | Наименование: Набор для колориметрического анализа гидроксипролина (HYP) / Hydroxyproline (HYP) Colorimetric Assay Kit.
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