Набор для анализа активности кислой фосфатазы (ACP) / Acid Phosphatase (ACP) Activity Assay Kit
- Detection instrument: Spectrophotometer(520 nm)
- Detection method: Colorimetric method
Manual
Detection principle
Acid phosphatase decomposes disodium phenyl phosphate under acidic conditions to produce free phenol and phosphoric acid. Phenol acts with 4-aminoantipyrine in alkaline solution, and oxidizes to a derivative of red quinone by potassium ferricyanide. The activity of the ACP can be calculated by measuring the OD value at 520 nm.
Performance characteristics
| Synonyms |
ACP |
| Sample type |
Serum,plasma,urine,tissue,cells |
| Sensitivity |
0.27 U/100 mL |
| Detection range |
0.27-40 U/100 mL |
| Detection method |
Colorimetric method |
| Assay type |
Enzyme Activity |
| Assay time |
70 min |
| Precision |
Average inter-assay CV: 7.100%Average intra-assay CV: 2.800% |
| Other instruments required |
Micropipettor, Incubator, Vortex mixer, Centrifuge |
| Other reagents required |
Normal saline (0.9% NaCl), PBS (0.01 M, pH 7.4) |
| Storage |
2-8℃ |
| Valid period |
12 months |
Информация для заказа| Область использования: | Производство: | Elabscience | | Метод: | | | Объем: | 96 тестов | | Кат. номер: | E-BC-K094-S | | Цена (с НДС 20%): | по запросу | В корзину  | Наименование: Набор для анализа активности кислой фосфатазы (ACP) / Acid Phosphatase (ACP) Activity Assay Kit.
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