- Detection method: Colorimetric method
Manual
Detection principle
Any compound that contains two -CONH2 in the molecule can react with alkaline copper solution to form a purple complex, which is known as the biuret reaction. Many peptide bonds (-CONH-) in protein molecules can perform this reaction, and the color degree of all kinds of proteins are essentially the same.
Performance characteristics
| Synonyms |
TP |
| Sample type |
Serum,plasma,tissue |
| Sensitivity |
0.373 g/L |
| Detection range |
0.373-80 g/L |
| Detection method |
Colorimetric method |
| Assay type |
Quantitative |
| Assay time |
50 min |
| Precision |
Average inter-assay CV: 2.600%Average intra-assay CV: 1.200% |
| Other instruments required |
Micropipettor, Incubator |
| Other reagents required |
Normal saline (0.9% NaCl), PBS (0.01 M, pH 7.4) |
| Storage |
Reagent 3: -20℃, others: 2-8℃ |
| Valid period |
12 months |
Dilution of sample
It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (0.373-80 g/L).
The recommended dilution factor for different samples is as follows (for reference only):
|
Sample type
|
Dilution factor
|
|
Porcine serum
|
2-4
|
|
Human serum
|
2-4
|
|
5% Mouse liver tissue homogenization
|
1
|
|
10% Epipremnum aureum tissue homogenization
|
1
|
|
5% Mouse heart tissue homogenization
|
1
|
Note: The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4).
