Набор для колориметрического анализа общего карбонила / Total Carbonyl Colorimetric Assay Kit

Carbonyl can react with 2,4-dinitrophenylhydrazine and produce a kind of reddish brown hydrazone compounds, which has a specific absorbance peak at 370 nm. The content of carbonyl can be calculated according to the absorbance value.

 

Performance characteristics

Sample type	Serum,plasma,,tissue
Sensitivity	0.94 μg/mL
Detection range	0.94-45 μg/mL
Detection method	Colorimetric method
Assay type	Quantitative
Assay time	50 min
Precision	Average inter-assay CV: 8.300%Average intra-assay CV: 4.400%
Other instruments required	Micropipettor, Vortex mixer
Other reagents required	Normal saline (0.9% NaCl), PBS (0.01 M, pH 7.4)
Storage	2-8℃
Valid period	12 months