- Detection method: Colorimetric method
Manual
Detection principle
Creatine kinase (CK) catalyze creatine phosphate and ADP to produce creatine and ATP. Hexokinase catalyze creatine and glucose to produce glucose-6-phosphate. Glucose-6-phosphate dehydrogenase (G-6-PD) catalyze glucose-6-phosphate and NADP+ to produce NADPH which have a maximum absorption peak at 340 nm. The CK activity can be calculated by measuring the OD values at 340 nm.
Performance characteristics
| Synonyms |
CK,Creatine phosphokinase |
| Sample type |
Serum,plasma,animal tissue,cells |
| Sensitivity |
3.7 U/L |
| Detection range |
3.7-160 U/L |
| Detection method |
Colorimetric method |
| Assay type |
Enzyme Activity |
| Assay time |
80 min |
| Precision |
Average inter-assay CV: 8.100%Average intra-assay CV: 5.200% |
| Other instruments required |
Micropipettor, Incubator, Vortex mixer |
| Other reagents required |
PBS (0.01 M, pH 7.4) |
| Storage |
2-8℃ |
| Valid period |
12 months |
Dilution of sample
It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (3.7-160 U/L).
The recommended dilution factor for different samples is as follows (for reference only):
|
Sample type
|
Dilution factor
|
|
Human serum
|
1
|
|
Human plasma
|
1
|
|
Mouse serum
|
1
|
|
Rat serum
|
1
|
|
HepG2 cells homogenization
|
1
|
|
10% Rat kidney tissue homogenization
|
1
|
|
10% Rat brain tissue homogenization
|
2-5
|
|
10% Rat liver tissue homogenization
|
2-10
|
Note: The diluent is PBS (0.01 M, pH 7.4).
