GOGAT catalysis the reaction that transfer the amino from glutamine to a-KG to form two molecules of glutamic acid using NADH as the electron donor. The decreasing rate of NADH that can be measured at 340 nm can reflect the activity of GOGAT.
Performance characteristics
Synonyms
GOGAT
Sample type
serum, plant and animal tissue
Sensitivity
8.84 U/L
Detection range
8.84–321.05 U/L
Detection method
Colorimetric method
Assay type
Enzyme Activity
Assay time
15 min
Precision
Average inter-assay CV: 5.800%Average intra-assay CV: 2.500%