Oxidative Hcy is converted into free Hcy, which can convert NADH to NAD+ through cyclic catalysis of enzymes, resulting in a decrease of OD value at 340 nm. The Hcy content in the sample is calculated by the rate of decrease.
Performance characteristics
Synonyms
Hcy
Sample type
serum (plasma) and urine
Sensitivity
1.95 μmol/L
Detection range
1.95-100.0 μmol/L
Detection method
Colorimetric method
Assay type
Quantitative
Assay time
25 min
Precision
Average inter-assay CV: 9%Average intra-assay CV: 1.700%