- Detection method: Colorimetric method
Manual
Detection principle
Pyruvic acid can react with chromogenic agent and the reaction product is reddish brown in alkaline solution. The depth of color is directly proportional to the pyruvate content. The pyruvate content can be calculated by measuring the OD value at 505 nm.
Performance characteristics
| Sample type |
Serum,plasma,tissue |
| Sensitivity |
0.006 μmol/mL |
| Detection range |
0.006-2.0 μmol/mL |
| Detection method |
Colorimetric method |
| Assay type |
Quantitative |
| Assay time |
60 min |
| Precision |
Average inter-assay CV: 1.500%Average intra-assay CV: 1.300% |
| Other instruments required |
Micropipettor, Vortex mixer, Incubator |
| Other reagents required |
Normal saline (0.9% NaCl), PBS (0.01 M, pH 7.4) |
| Storage |
2-8℃ |
| Valid period |
12 months |
Dilution of sample
It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (0.006-2.0 μmol/mL).
The recommended dilution factor for different samples is as follows (for reference only):
|
Sample type
|
Dilution factor
|
|
Human serum
|
1
|
|
Mouse serum
|
1
|
|
Mouse plasma
|
1
|
|
10% Mouse liver tissue homogenization
|
1
|
|
10% Rat kidney tissue homogenization
|
1
|
|
10% Rat heart tissue homogenization
|
1
|
Note: The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4).
