Reactive Oxygen Species (ROS) Fluorometric Assay Kit (to test fresh tissue and alive cell samples)
Reactive Oxygen Species (ROS)F
Specification:96T
Measuring instrument:Fluorescence Microplate reader, Fluorescence
microscope, Flow Cytometry luorometric Assay Kit (Green)
Intended use This kit can be used to measure reactive oxygen species (ROS) in fresh tissue and
alive cell samples.
Detection principle DCFH-DA (2,7-dichlorofuorescin diacetate) is a fluorescent probe without
fluorescence that can freely cross the membrane. After entering the cell, it can be
hydrolyzed by intracellular esterase to form DCFH (dichlorofluorescin). In the presence of reactive oxygen species (ROS), DCFH is oxidized to DCF (dichlorofluorescein) which is a strong green fluorescent substance that cannot
penetrate the cell membrane. DCF has a maximum wave peak near the excitation wavelength of 502 nm and the emission wavelength of 525 nm, and the intensity is proportional to the level of intracellular reactive oxygen species.
Информация для заказа
Область использования: | Производство: | Puda Scientific |
Метод: | |
Объем: | |
Кат. номер: | PD-KE0138 |
Цена (с НДС 20%): | по запросу | В корзину |
Наименование: Reactive Oxygen Species (ROS) Fluorometric Assay Kit (to test fresh tissue and alive cell samples). Примечание: |