Reactive Oxygen Species (ROS) Fluorometric Assay Kit (to test alive cell samples)
Specification：96T
Measuring instrument：Fluorescence Microplate reader, Fluorescence microscope, Flow Cytometry
Intended use Reactive Oxygen Species (ROS) Fluorometric Assay Kit (Red)
This kit can be used to measure reactive oxygen species (ROS) in alive cell samples.
Detection principle
Reactive oxygen species (ROS) refers to the general term of substances that are
composed of oxygen in the body or in the natural environment and contain oxygen
and are active in nature: there is mainly a kind of excited oxygen molecule,
namely singlet oxygen molecule or singlet oxygen molecule. Three oxygencontaining free radicals, namely superoxide anion radicals, hydroxyl radicals, and hydroperoxyradicals, two peroxides, namely hydrogen peroxide and lipid
peroxide, and a nitrogen-containing oxide. Dihydroethidium can cross the cell
membrane and enter the cell to bind to DNA or RNA, forming hydroethidium to
produce red fluorescence. Dihydroethidium is mainly oxidized by superoxide
anion type reactive oxygen species in cells.
Dihydroethidium itself is blue fluorescence with a maximum excitation wavelength
of 370 nm and a maximum emission wavelength of 420 nm. After dehydrogenation,
dihydroethidium binds to RNA or DNA to produce red fluorescence with an
excitation wavelength of 300 nm or 518 nm and an emission wavelength of 610 nm.
Информация для заказа
| Область использования: | Производство: | Puda Scientific |
| Метод: | |
| Объем: | |
| Кат. номер: | PD-FE0005 |
| Цена (с НДС 20%): | по запросу | В корзину  |
Наименование: Reactive Oxygen Species (ROS) Fluorometric Assay Kit (to test alive cell samples) .
Примечание: |
