Набор CatchExo Exosome Isolation Kit - for Serum для выделения экзосом из сыворотки (до 250 мкл), 120 реакций (BiOptic)
Набор для выделения экзосом CatchExo Exosome Isolation Kit - for Serum основан на методе осаждения и обеспечивает простое и быстрое выделение экзосом всего из 250 мкл человеческой сыворотки. Протокол удобен для пользователя, экономит время и не требует высокоскоростной центрифуги. Из очищенных с помощью набора экзосом можно затем выделять нуклеиновые кислоты.
Kit Description
The CatchExo Exosome Isolation Kit - for Serum is based on precipitation method and provide an easy
and quick approach to enrich exosome for a good performance in the subsequent nucleic acid
extraction, from as little as 250 μl of human serum. The protocol is user friendly, timesaving (around 1
hour), and does not require high speed centrifuge.
Kit Storage
The CatchExo Exosome Isolation Kits are shipped at room temperature and should be stored at 4°C
upon arrival. Properly stored kits are stable for 1 year from the date received.
Sample Pretreatment
The suggested human sample is serum or body fluid (not tested), otherwise the exosome pellet
would become too viscous to re-suspend. For plasma, it’s better to do thrombin pretreatment to get
serum. Please contact CatchGene Co., Ltd. for thrombin related product information.
For Serum Sample
1. Serum should be stored at -80°C in aliquots until further processing.
2. Before the analyses, thawed serum samples were centrifuged at 3,000g for 15 min to remove cells
and cell debris.
General Protocol
1. Pipette 250 μl serum sample into 1.5 ml micro-centrifuge tube (not provided) and add 100 μl EV
Precipitation Buffer. Mix by repeat pipetting then briefly spin down.
2. Incubate the mixture on ice for 30 minutes.
3. Centrifuge at 1,500 x g for 30 minutes at 4°C.
4. Aspirate the supernatant to eliminate any residual EV Precipitation Buffer. Briefly respin to collect
and remove residual supernatant.
5. Resuspend the exosome pellet in 100-300ul Resuspension Buffer according to the downstream
application.
6. Continue miRNA or other nucleic acid extraction with the resuspended pellet using coordinated
CatchGene Kit or can be stored at -20°C no more than two weeks prior to nucleic acid isolation
Информация для заказа
| Область использования: | Производство: | --- |
| Метод: | |
| Объем: | |
| Кат. номер: | EX10120 |
| Цена (с НДС 20%): | по запросу | В корзину  |
Наименование: Набор CatchExo Exosome Isolation Kit - for Serum для выделения экзосом из сыворотки (до 250 мкл), 120 реакций (BiOptic).
Примечание: |
