Uniprot No# | P0DTC9 |
Специфичность | SARS-CoV-2 |
Источник | E.coli |
Информация о тегах | N-terminal 6xHis-tagged |
Регион экспрессии | 1-419aa |
Описание последовательности | Full Length |
Последовательность белка-мишени | MSDNGPQNQRNAPRITFGGPSDSTGSNQNGERSGARSKQRRPQGLPNNTASWFTALTQHGKEDLKFPRGQGVPINTNSSPDDQIGYYRRATRRIRGGDGKMKDLSPRWYFYYLGTGPEAGLPYGANKDGIIWVATEGALNTPKDHIGTRNPANNAAIVLQLPQGTTLPKGFYAEGSRGGSQASSRSSSRSRNSSRNSTPGSSRGTSPARMAGNGGDAALALLLLDRLNQLESKMSGKGQQQQGQTVTKKSAAEASKKPRQKRTATKAYNVTQAFGRRGPEQTQGNFGDQELIRQGTDYKHWPQIAQFAPSASAFFGMSRIGMEVTPSGTWLTYTAAIKLDDKDPNFKDQVILLNKHIDAYKTFPPTEPKKDKKKKADETQALPQRQKKQQTVTLLPAADLDDFSKQLQQSMSSADSTQA |
Молекулярный вес | 48.0 kDa |
Чистота | Greater than 90% as determined by SDS-PAGE. |
ELISA-антитело-связывающая активность | Measured by its binding ability in a functional ELISA. Immobilized SARS-CoV-2-N at 5 μg/ml can bind SARS-CoV-2-N Antibody (CSB-RA33255A1GMY), the EC50 of SARS-CoV-2-N protein is 43.50-118.4 ng/ml. |
Форма | Lyophilized powder |
Буфер | Lyophilized from a 0.2 μm filtered 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0 |
Восстановление | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference. |
ELISA-активность связывания антител
|
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
|
Positive WB detected in: His tag-tagged SARS-CoV-2 nucleocapsid recombinant protein from E. Coli (CSB-EP3325GMY)
SARS-CoV-2 nucleocapsid antibody at 1:1000 (CSB-RA33255A1GMY)
Secondary
Peroxidase-Affinipure Goat Anti-Human IgG, Fc? Fragment Specific at 1/20000 dilution
Predicted band size: 48 kDa
Observed band size: 55 kDa
|
Ссылка на страницу на сайте производителя | ссылка |
| |