Uniprot No# | P0DTC2 |
Специфичность | SARS-CoV-2 |
Источник | Yeast |
Информация о тегах | N-terminal 6xHis-sumostar-tagged |
Регион экспрессии | 319-541aa |
Описание последовательности | Partial (S1-RBD) |
Последовательность белка-мишени | RVQPTESIVRFPNITNLCPFGEVFNATRFASVYAWNRKRISNCVADYSVLYNSASFSTFKCYGVSPTKLNDLCFTNVYADSFVIRGDEVRQIAPGQTGKIADYNYKLPDDFTGCVIAWNSNNLDSKVGGNYNYLYRLFRKSNLKPFERDISTEIYQAGSTPCNGVEGFNCYFPLQSYGFQPTNGVGYQPYRVVVLSFELLHAPATVCGPKKSTNLVKNKCVNF |
Чистота | Greater than 85% as determined by SDS-PAGE. |
ELISA-антитело-связывающая активность | Measured by its binding ability in a functional ELISA. Immobilized SARS-CoV-2-S1-RBD at 2 μg/ml can bind SARS-CoV-2-S Antibody (CSB-RA33245A1GMY), the EC50 of SARS-CoV-2-S1-RBD protein is 19.60-39.42 ng/ml. |
Активность взаимодействия лигандов | Testing in progress. It is expected to be updated on April 22, 2020. |
Форма | Lyophilized powder |
Буфер | Tris/PBS-based buffer, 6% Trehalose, pH 8.0 |
Восстановление | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference. |
ELISA-активность связывания антител
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(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
Predicted band size: 38.2 kDa
Observed band size:
(a) 68 kDa before EndoH Digestion
(b) 38 kDa after EndoH Digestion
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