- Product Name
- COVID-19 Spike Protein(RBD) ELISA Kit
- Alias
- Spike Protein ELISA Kit, RBD) ELISA Kit
- Catalogue No.
- EU3128
- Size
- 48T/96T
- Species
- Coronavirus (COVID-19, SARS-CoV-2, 2019-nCoV, Novel Coronavirus Spike Glycoprotein,RBD, SARS-CoV-2 S1)
- UniProt No.
- P0DTC2
- Sample Type
- Serum, Plasma, Cell Culture Supernatant, cell or tissue lysate, Other liquid samples
- Detection Method
- Sandwich ELISA, Double Antibody
- Detection Wavelength
- OD450
- Reaction Duration
- 4 hours
- Range
- 23.438-1500pg/ml
- Sensitivity
- 14.063pg/ml
- Storage
- 2-8°C(Sealed), Don't cryopreserve.
- Specificity
- Specifically binds with COVID-19-S (RBD) , no obvious cross reaction with other analogues.
- Required Instruments and Reagents
-
- Microplate reader (wavelength: 450nm)
- 37°C incubator (CO2 incubator for cell culture is not recommenced.)
- Automated plate washer or multi-channel pipette/5ml pipettor (for manual washing purpose)
- Precision single (0.5-10μL, 5-50μL, 20-200μL, 200-1000μL) and multi-channel pipette with disposable tips(Calibration is required before use.)
- Sterile tubes and Eppendorf tubes with disposable tips
- Absorbent paper and loading slot
- Deionized or distilled water
- Standard Curve
-
This product is detected by QC department and meets performance required in the manual. (Laboratory Humidity: 20%-60%; Temperature: 18°C -25°C; Equilibrate TMB substrate to 37°C before staining. After adding into the ELISA wells, incubate for 15min at 37°C in dark.)
Due to different assay environments and operations, assay data below and standard curve are provided for reference. Experimenters should establish standard curve according to their own assay.
| STD.(pg/ml) |
OD-1 |
OD-2 |
Average |
| 0 |
0.06 |
0.063 |
0.061 |
| 23.438 |
0.16 |
0.168 |
0.163 |
| 46.875 |
0.272 |
0.286 |
0.278 |
| 93.75 |
0.47 |
0.494 |
0.48 |
| 187.5 |
0.725 |
0.762 |
0.74 |
| 375 |
1.235 |
1.298 |
1.26 |
| 750 |
1.787 |
1.878 |
1.823 |
| 1500 |
2.061 |
2.166 |
2.103 |
- Recovery
-
Add a certain amount of COVID-19-S (RBD) into the sample. Calculate the recovery by comparing the measured value with the expected amount of COVID-19-S (RBD) in the sample.
| Sample Type |
Recovery Range(%) |
Average(%) |
| serum(n=10) |
85-103 |
92 |
| EDTA plasma(n=10) |
89-101 |
97 |
| Heparin plasma(n=10) |
86-104 |
99 |
- Linearity
-
Dilute the sample with a certain amount of COVID-19-S (RBD) at 1:2, 1:4 and 1:8 to get the recovery range.
| Sample Type |
1:2 |
1:4 |
1:8 |
| serum(n=10) |
90-101% |
89-102% |
85-97% |
| EDTA plasma(n=10) |
83-91% |
82-97% |
82-96% |
| Heparin plasma(n=10) |
84-99% |
88-99% |
85-99% |
- Precision(%)
-
Intra-assay Precision: samples with low, medium and high concentration are tested 20 times on the same plate.
Inter-assay Precision: samples with low, medium and high concentration are tested 20 times on three different plates.
| Item |
Intra-assay Precision |
Inter-assay Precision |
| Sample |
1 |
2 |
3 |
1 |
2 |
3 |
| n |
20 |
20 |
20 |
20 |
20 |
20 |
| Mean (pg/ml) |
46.42 |
194 |
756 |
45.91 |
187 |
748 |
| Standard deviation |
2.3 |
11.52 |
39.54 |
2.85 |
8.75 |
38 |
| CV(%) |
4.95 |
5.94 |
5.23 |
6.2 |
4.68 |
5.08 |
- Stability
-
Perform the stability test for the sealed kit at 37°C and 2-8°C and get relevant data.
| ELISA kit(n=5) |
37°C for 1 month |
2-8°C for 6 months |
| Average(%) |
80 |
95-100 |
