| Объем | 50 мкл |
| Синонимы | Caspase-9, Apoptotic protease Mch-6, Apoptotic protease-activating factor 3, APAF-3, ICE-like apoptotic protease 6, ICE-LAP6, Caspase-9 subunit p35, Caspase-9 subunit p10, CASP9, MCH6 |
| Тип антител | Recombinant Antibody |
| Species | Human |
| UniProt ID | P55211 |
| Иммуноген | A synthesized peptide derived from human CASP9 |
| Видовая специфичность | Human |
| Применение | ELISA, WB, IF, FC, Recommended dilution: WB:1:500-1:5000, IF:1:20-1:200 |
| Клональность | Monoclonal |
| Изотип | Rabbit IgG |
| Коньюгат | Non-conjugated |
| Буффер | Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Форма | Liquid |
| Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Метод очистки | Affinity-chromatography |
| Области исследований | Cell Biology |
| Аббревиатура | Caspase-9 |
| Примечание | Involved in the activation cascade of caspases responsible for apoptosis execution. Binding of caspase-9 to Apaf-1 leads to activation of the protease which then cleaves and activates caspase-3. Promotes DNA damage-induced apoptosis in a ABL1/c-Abl-dependent manner. Proteolytically cleaves poly(ADP-ribose) polymerase (PARP). |
| Ссылка на страницу на сайте производителя | ссылка |
Western Blot
Positive WB detected in: HL-60 whole cell lysate, LO2 whole cell lysate
All lanes: CASP9 antibody at 1.8µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 47, 31, 18, 37 KDa
Observed band size: 47 KDa
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Immunofluorescence staining of HepG2 cells with CSB-RA004555A0HU at 1:60, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).
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Overlay histogram showing K562 cells stained with CSB-RA004555A0HU (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then permeabilized with 0.3% Triton X-100 for 2 min. The cells were then incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by primary antibody for 1 h at 4°C. The secondary antibody used was FITC goat anti-rabbit IgG (H+L) at 1/200 dilution for 1 h at 4°C. Control antibody (green line) was used under the same conditions. Acquisition of >10,000 events was performed.
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Наименование: CASP9 Antibody.