Объем | 100 мкл |
Синонимы | Mitofusin-1 (EC 3.6.5.-) (Fzo homolog) (Transmembrane GTPase MFN1), MFN1 |
Клональность | Polyclonal Antibody |
Организм | Human |
uniprot | Q8IWA4 |
Иммуноген | Recombinant Human Mitofusin-1 protein (622-741AA) |
Источник | Rabbit |
Видовая специфичность | Human |
Применение | ELISA, WB, IHC, IP, Recommended dilution: WB:1:1000-1:5000, IHC:1:20-1:200, IP:1:200-1:2000 |
Примечание | Essential transmembrane GTPase, which mediates mitochondrial fusion. Fusion of mitochondria occurs in many cell types and constitutes an important step in mitochondria morphology, which is balanced between fusion and fission. MFN1 acts independently of the cytoskeleton. Overexpression induces the formation of mitochondrial networks. |
Клональность1 | Polyclonal |
Изотип | IgG |
Коньюгат | Non-conjugated |
Буффер | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
Форма | Liquid |
Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
Метод очистки | Antigen Affinity Purified |
Абревеатура | Mitofusin-1 |
Области исследований | Neuroscience, Tags & Cell Markers, Metabolism, Signal transduction |
Ссылка на страницу на сайте производителя | ссылка |
Western blot
All lanes: MFN1 antibody at 1.1µg/ml
Lane 1: Human placenta tissue
Lane 2: Jurkat whole cell lysate
Lane 3: Hela whole cell lysate
Lane 4: K562 whole cell lysate
Secondary
Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 85, 42, 72 kDa
Observed band size: 85 kDa
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Immunohistochemistry of paraffin-embedded human testis tissue using CSB-PA812879ESR2HU at dilution of 1:100
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Immunoprecipitating MFN1 in Jurkat whole cell lysate
Lane 1: Rabbit monoclonal IgG (1µg) instead of CSB-PA812879ESR2HU in Jurkat whole cell lysate.
For western blotting, a HRP-conjugated light chain specific antibody was used as the Secondary antibody (1/50000)
Lane 2: CSB-PA812879ESR2HU (4µg) + Jurkat whole cell lysate (500µg)
Lane 3: Jurkat whole cell lysate (20µg)
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