| Объем | 100 мкл |
| Синонимы | PKB beta, Protein kinase B, RAC-PK-beta |
| Клональность | Polyclonal Antibody |
| Организм | Human |
| uniprot | P31751 |
| Иммуноген | Peptide sequence around phosphorylation site of serine 474 (Q-F-S(p)-Y-S) derived from Human Akt2. |
| Источник | Rabbit |
| Видовая специфичность | Human, Mouse, Rat |
| Применение | ELISA, WB, IHC,WB:1:500-1:1000, IHC:1:50-1:100 |
| Примечание | <p>General protein kinase capable of phosphorylating several known proteins. <br /><br />Sun M, et al. (2001) Cancer Res, 61(16): 5985-91. <br />Yuan ZQ, et al. (2000) Oncogene, 19(19): 2324-30. <br />Meier R, et al. (1997) J Biol Chem, 272(48): 30491-7.</p> |
| Клональность1 | Polyclonal |
| Изотип | IgG |
| Коньюгат | Non-conjugated |
| Буффер | Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Форма | liquid |
| Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Метод очистки | Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide. |
| Абревеатура | Phospho-Ser474 |
| Области исследований | Cancer,Cardiovascular,Metabolism,Signal transduction |
| Ссылка на страницу на сайте производителя | ссылка |
Western blot analysis of extracts from 293 cells untreated(lane 1) or treated with serum(lane 2) using Akt2(Phospho-Ser474) Antibody.
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Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using Akt2(Phospho-Ser474) Antibody(left) or the same antibody preincubated with blocking peptide(right).
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Western blot analysis of extracts from 293 cells, treated with serum or calf intestinal phosphatase (CIP), using Akt2 (Phospho-Ser474) Antibody.
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Наименование: Phospho-AKT2 (Ser474) Antibody.