| Объем | 100 мкл |
| Синонимы | Bbc2 |
| Клональность | Polyclonal Antibody |
| Организм | Human |
| uniprot | Q61337 |
| Иммуноген | Peptide sequence around phosphorylation site of serine 112 (H-S-S(p)-Y-P) derived from Mouse BAD. |
| Источник | Rabbit |
| Видовая специфичность | Human, Mouse, Rat |
| Применение | ELISA, WB, IHC,WB:1:500-1:1000, IHC:1:50-1:100 |
| Примечание | <p>The protein encoded by BAD gene is a member of the BCL-2 family. BCL-2 family members are known to be regulators of programmed cell death. This protein positively regulates cell apoptosis by forming heterodimers with BCL-xL and BCL-2, and reversing their death repressor activity. Proapoptotic activity of this protein is regulated through its phosphorylation. Protein kinases AKT and MAP kinase, as well as protein phosphatase calcineurin were found to be involved in the regulation of this protein. Alternative splicing of this gene results in two transcript variants which encode the same isoform. <br /><br />Zhang B, et al. (2004). Mol Cell Biol.24 (14): 6205-6214. <br />Rice PL, et al. (2003). Cancer Res.63 (3): 616-620. <br />Wang XQ, et al. (2001). J Biol Chem.276 (48): 44504-44511.</p> |
| Клональность1 | Polyclonal |
| Изотип | IgG |
| Коньюгат | Non-conjugated |
| Буффер | Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Форма | liquid |
| Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Метод очистки | Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide. |
| Абревеатура | Phospho-Ser112 |
| Области исследований | Cancer,Cell biology,Metabolism |
| Ссылка на страницу на сайте производителя | ссылка |
Western blot analysis of extracts from cos7 cells untreated or treated with EGF using BAD(Phospho-Ser112) Antibody
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Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using BAD(Phospho-Ser112) Antibody(left) or the same antibody preincubated with blocking peptide(right).
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Наименование: Phospho-Bad (Ser112) Antibody.