| Объем | 100 мкг |
| Синонимы | Receptor-interacting serine/threonine-protein kinase 1 (EC 2.7.11.1) (Cell death protein RIP) (Receptor-interacting protein 1) (RIP-1) (Serine/threonine-protein kinase RIP), Ripk1, Rinp Rip |
| Клональность | Polyclonal Antibody |
| Организм | Mouse |
| uniprot | Q60855 |
| Иммуноген | Recombinant Mouse Receptor-interacting serine/threonine-protein kinase 1 protein (1-656AA) |
| Источник | Rabbit |
| Видовая специфичность | Mouse, Human |
| Применение | ELISA, IP, Recommended dilution: IP:1:200-1:2000 |
| Примечание | Serine-threonine kinase which transduces inflammatory and cell-death signals (programmed necrosis) following death receptors ligation, activation of pathogen recognition receptors (PRRs), and DNA damage. Upon activation of TNFR1 by the TNF-alpha family cytokines, TRADD and TRAF2 are recruited to the receptor. Phosphorylates DAB2IP at 'Ser-728' in a TNF-alpha-dependent manner, and thereby activates the MAP3K5-JNK apoptotic cascade. Ubiquitination by TRAF2 via 'Lys-63'-link chains acts as a critical enhancer of communication with downstream signal transducers in the mitogen-activated protein kinase pathway and the NF-kappa-B pathway, which in turn mediate downstream events including the activation of genes encoding inflammatory molecules. Polyubiquitinated protein binds to IKBKG/NEMO, the regulatory subunit of the IKK complex, a critical event for NF-kappa-B activation. Interaction with other cellular RHIM-containing adapters initiates gene activation and cell death. RIPK1 and RIPK3 association, in particular, forms a necrosis-inducing complex (By similarity). Interacts with ARHGEF2 (By similarity). |
| Клональность1 | Polyclonal |
| Изотип | IgG |
| Коньюгат | Non-conjugated |
| Буффер | Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
| Форма | Liquid |
| Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Метод очистки | >95%, Protein G purified |
| Абревеатура | Receptor-interacting serine/threonine-protein kinase 1 |
| Области исследований | Cancer, Cell biology, Signal transduction |
| Ссылка на страницу на сайте производителя | ссылка |
Immunoprecipitating Ripk1 in K562 whole cell lysate
Lane 1: Rabbit control IgG (1µg) instead of CSB-PA720181LA01MO in K562 whole cell lysate.
For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
Lane 2: CSB-PA720181LA01MO (6µg) + K562 whole cell lysate (500µg)
Lane 3: K562 whole cell lysate (10µg)
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Наименование: Ripk1 Antibody.