Phospho-AKT1/AKT3 (Tyr437/434) AntibodyСпецификация| Объем | 100 мкл | | Синонимы | EC 2.7.11.1, RAC-PK-alpha, Protein kinase B, PKB, C-AKT | | Клональность | Polyclonal Antibody | | Организм | Human | | uniprot | P31749/Q9Y243 | | Иммуноген | Peptide sequence around phosphorylation site of tyrosine 437 (T-R-Y(p)-F-D) derived from Human AKT1/3. | | Источник | Rabbit | | Видовая специфичность | Human, Mouse, Rat | | Применение | ELISA, WB, IHC,WB:1:500-1:3000, IHC:1:50-1:100 | | Примечание | AKT1 is one of 3 closely related serine/threonine-protein kinases (AKT1, AKT2 and AKT3) called the AKT kinase, and which regulate many processes including metabolism, proliferation, cell survival, growth and angiogenesis. This is mediated through serine and/or threonine phosphorylation of a range of downstream substrates. Over 100 substrate candidates have been reported so far, but for most of them, no isoform specificity has been reported. AKT is responsible of the regulation of glucose uptake by mediating insulin-induced translocation of the SLC2A4/GLUT4 glucose transporter to the cell surface. Phosphorylation of PTPN1 at 'Ser-50' negatively modulates its phosphatase activity preventing dephosphorylation of the insulin receptor and the attenuation of insulin signaling. | | Клональность1 | Polyclonal | | Изотип | IgG | | Коньюгат | Non-conjugated | | Буффер | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. | | Форма | liquid | | Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. | | Метод очистки | Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide. | | Абревеатура | Phospho-Tyr437/434 | | Области исследований | Epigenetics and Nuclear Signaling,Neuroscience,Cancer,Metabolism,Signal transduction | | Ссылка на страницу на сайте производителя | ссылка | Western blot analysis of extracts from K562 cells, treated with insulin (0.01U/ml, 15mins), using AKT1/3 (Phospho-Tyr437/434) antibody. The lane on the right is treated with the synthesized peptide.
 | Immunohistochemistry analysis of paraffin-embedded human brain tissue using AKT1/3 (Phospho-Tyr437/434) antibody. The picture on the right is treated with the synthesized peptide.
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Информация для заказа| Область использования: | Производство: | Cusabio | | Метод: | Антитела | | Объем: | 100 мкл | | Кат. номер: | CSB-PA038073 | | Цена (с НДС 20%): | по запросу | В корзину  |  Наименование: Phospho-AKT1/AKT3 (Tyr437/434) Antibody.
Примечание: дополнительная информация (на английском языке). |
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