Recombinant human Methylated-DNA--protein-cysteine methyltransferase protein

Relevance: Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a suicide reaction: the enzyme is irreversibly inactivated.
Product Info:
Source:
Purity: 90%
Image:
Tested applications: SDS-PAGE, ELISA
Storage Buffer: 6M guanidine hydrochloride, 20mM Tris
Storage: Store at -20?, for extended storage, conserve at -20? or -80?.
Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4? for up to one week.
AA sequence:
Referrences: [1] "Isolation and structural characterization of a cDNA clone encoding the human DNA repair protein for O6-alkylguanine." Tano K., Shiota S., Collier J., Foote R.S., Mitra S. Proc. Natl. Acad. Sci. U.S.A. 87:686-690(1990) [PubMed: 2405387] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 1-8. [2] "cDNA cloning and chromosomal assignment of the human O6-methylguanine-DNA methyltransferase. cDNA expression in Escherichia coli and gene expression in human cells." Rydberg B., Spurr N., Karran P. J. Biol. Chem. 265:9563-9569(1990) [PubMed: 2188979] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. [3] "Purification, structure, and biochemical properties of human O6-methylguanine-DNA methyltransferase." Koike G., Maki H., Takeya H., Hayakawa H., Sekiguchi M. J. Biol. Chem. 265:14754-14762(1990) [PubMed: 2394694] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Alias: 6-O-methylguanine-DNA methyltransferase,MGMT,O-6-methylguanine-DNA-alkyltransferase.

Информация для заказа

Область использования: Рекомбинантные белки Cusabio	Производство:	Cusabio
	Метод:
	Объем:	200 мкг
	Кат. номер:	CSB-RP118574h
Цена (с НДС 20%):	по запросу	В корзину
Наименование: Recombinant human Methylated-DNA--protein-cysteine methyltransferase protein. Примечание: дополнительная информация (на английском языке).