Recombinant human Osteopontin protein
Relevance: Binds tightly to hydroxyapatite. Appears to form an integral part of the mineralized matrix. Probably important to cell-matrix interaction. Acts as a cytokine involved in enhancing production of interferon-gamma and interleukin-12 and reducing production of interleukin-10 and is essential in the pathway that leads to type I immunity By similarity.
Product Info:
Source:
Purity: 90%
Image:
Tested applications: SDS-PAGE, ELISA
Storage Buffer: 20mM Tris-Hcl, 0.5M NaCl, 10% glycerin, PH 8.0,200 mM Imidazole
Storage: Store at -20?, for extended storage, conserve at -20? or -80?.
Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4? for up to one week.
AA sequence:
Referrences: [1] "The cDNA and derived amino acid sequence for human osteopontin." Kiefer M.C., Bauer D.M., Barr P.J. Nucleic Acids Res. 17:3306-3306(1989) [PubMed: 2726470] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM A). [2] "cDNA cloning, mRNA distribution and heterogeneity, chromosomal location, and RFLP analysis of human osteopontin (OPN)." Young M.F., Kerr J.M., Termine J.D., Wewer U.M., Wang M.G., McBride O.W., Fisher L.W. Genomics 7:491-502(1990) [PubMed: 1974876] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM B). [3] "Inhibition of calcium oxalate crystal growth in vitro by uropontin: another member of the aspartic acid-rich protein superfamily." Shiraga H., Min W., VanDusen W.J., Clayman M.D., Miner D., Terrell C.H., Sherbotie J.R., Foreman J.W., Przysiecki C., Neilson E.G., Hoyer J.R. Proc. Natl. Acad. Sci. U.S.A. 89:426-430(1992) [PubMed: 1729712] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM A).
Alias: Bone sialoprotein 1,Nephropontin,Secreted phosphoprotein 1,SPP-1,Urinary stone protein,Uropontin.
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