In acidic condition, the copper ion in the sample react with complexing agent to form a purple complex which has a maximum absorption peak at 580 nm. And copper ion content can be calculated indirectly by measuring the OD value at 580 nm.
Performance characteristics
Synonyms
Cu
Sample type
cell
Sensitivity
0.18 μmol/L
Detection range
0.18-5 μmol/L
Detection method
Colorimetric method
Assay type
Quantitative
Assay time
10 min
Precision
Average inter-assay CV: 3.100%Average intra-assay CV: 3%