- Detection method: Colorimetric method
Manual
Detection principle
The zinc ion in the sample react with 5-Br-PADAP to produce the colored complex. The depth of color is directly proportional to the concentration of zinc ion. Zinc ion content can be calculated by measuring the OD values at 560 nm.
Performance characteristics
| Synonyms |
Zn |
| Sample type |
Serum,plasma,urine,milk |
| Sensitivity |
0.418 μmol/L |
| Detection range |
0.748 -46.2 μmol/L |
| Detection method |
Colorimetric method |
| Assay type |
Quantitative |
| Assay time |
20 min |
| Precision |
Average inter-assay CV: 4%Average intra-assay CV: 2.700% |
| Other instruments required |
Micropipettor, Vortex mixer |
| Other reagents required |
Deionized water |
| Storage |
2-8℃ |
| Valid period |
12 months |
Dilution of sample
It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (0.748 -46.2 μmol/L).
The recommended dilution factor for different samples is as follows (for reference only):
|
Sample type
|
Dilution factor
|
|
Human urine
|
1
|
|
Human serum
|
1
|
|
Human milk
|
1
|
|
Rat serum
|
1
|
Note: The diluent is deionized water.
