β-galactosidase activated by sodium ions can catalyze o-nitrophenol to produce glycoside and o-nitrophenol. The increase of absorbance is determined at 405 nm, and the content of sodium ion is calculated indirectly.
Performance characteristics
Sample type
serum, plasma, tissue
Sensitivity
0.02 mmol/L
Detection range
0.02 - 20 mmol/L
Detection method
Colorimetric method
Assay type
Quantitative
Assay time
15 min
Precision
Average inter-assay CV: 8.400%Average intra-assay CV: 2.200%