- Detection method: Colorimetric method
Manual
Detection principle
The excess iron is added to the serum to bind all the ferritin in the serum, and the excess iron is adsorbed by adding the iron adsorbent. The iron bind with the ferritin is separated from the protein by the action of acid solution and reductant. Fe3+ in serum is reduced to Fe2+, Fe2+ binds with bipyridine to form pink complex. In a certain range, the amount of TIBC is positively correlated with the depth of color. The iron content measured is, minus serum iron value, which is called unsaturated iron binding force. Total iron binding capacity minus serum iron value is unsaturated iron binding capacity (UIBC).
Performance characteristics
| Synonyms |
TIBC |
| Sample type |
Serum |
| Sensitivity |
0.14 mg/L |
| Detection range |
0.31-50 mg/L |
| Detection method |
Colorimetric method |
| Assay type |
Quantitative |
| Assay time |
20 min |
| Precision |
Average inter-assay CV: 2.300%Average intra-assay CV: 1.500% |
| Other instruments required |
Micropipettor, Water bath, Incubator, Vortex mixer, Centrifuge |
| Storage |
2-8℃ |
| Valid period |
12 months |
Dilution of sample
It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (0.31-50 mg/L).
The recommended dilution factor for different samples is as follows (for reference only):
|
Sample type
|
Dilution factor
|
|
Human serum
|
1
|
|
Rat serum
|
1
|
|
Porcine serum
|
1
|
|
Rabbit serum
|
1
|
|
Chicken serum
|
1
|
|
Machin serum
|
1
|
Note: The diluent is double distilled water.
