PepTivator SARS-CoV-2 Prot_S

PepTivator®

SARS-CoV-2 Prot_S is a pool of lyophilized peptides, consisting mainly of 15-mer sequences with 11 amino acids overlap, covering the immunodominant sequence domains of the surface (or spike) glycoprotein (“S”) of SARS-Coronavirus 2 (GenBank MN908947.3, Protein QHD43416.1). The PepTivator SARS-CoV-2 Prot_S contains the sequence domains aa 304-338, 421-475, 492-519, 683-707, 741-770, 785-802, and 885 – 1273 (sequence end).

In vitro

stimulation of antigen-specific T cells with PepTivator Peptide Pools causes the secretion of effector cytokines and the up-regulation of activation markers, which then allow the detection and isolation of antigen-specific T cells.

Background information

SARS-CoV-2 (severe acute respiratory syndrome coronavirus 2), also known as novel coronavirus 2019-nCoV, causes fever, severe respiratory illness and can lead to life threatening pneumonia. The first cases of this disease, termed COVID-19 for coronavirus disease 2019, have been detected in December 2019 in Wuhan, China.

SARS-CoV-2 Prot_S stands for the surface glycoprotein of SARS-CoV-2, the “spike protein”. This protein is responsible for the recognition and binding of the coronavirus to the host cell. Once SARS-CoV-2 has bound to the ACE2 receptor of the host cell, fusion of viral envelope and host cell membrane initiate, which enables the viral genome to enter the host cell. Thus, the spike proteins are crucial for the infection of cells with coronaviruses and have been suggested as possible target for vaccine development.

Applications

PepTivator®

SARS-CoV-2 Prot_S – research grade has been specifically developed for

i n vitro

stimulation of SARS-CoV-2– specific T cells. Peptides of 15 amino acids in length and 11 amino acids overlap represent an optimized solution for stimulating both CD4+

and CD8+

T cells in various applications, including:

Detection and analysis of SARS-CoV-2–specific CD4⁺ and CD8⁺ effector/memory T cells in PBMCs by MACS^® Cytokine Secretion Assays, intracellular cytokine staining, or other technologies.
Isolation of viable SARS-CoV-2–specific CD4⁺ T cells with the CD154 MicroBead Kit, or of viable CD4⁺and CD8⁺ T cells using MACS Cytokine Secretion Assay – Cell Enrichment and Detection Kits. Subsequently, cells can be expanded for generation of T cell lines.
Generation of SARS-CoV-2–specific CD4⁺ and CD8⁺ effector/ memory T cells from naive T cell populations.
Pulsing of antigen-presenting cells, e.g. for research on dendritic cell vaccination.

More information regarding the work with antigen-specific T cells can be found in the T cell application section.

130-126-700

PepTivator SARS-CoV-2 Prot_S

for stimulation of 1×108 cells

Информация для заказа

Область использования: Диагностика коронавируса SARS-CoV-2 (COVID-19) Пептидные пулы для исследования Т-клеток, специфичных для SARS-CoV-2 Miltenyi Biotec	Производство:	Miltenyi Biotec
	Метод:
	Объем:	60 nmol
	Кат. номер:	130-126-701
Цена (с НДС 20%):	по запросу	В корзину
Наименование: PepTivator SARS-CoV-2 Prot_S. Примечание: For research use only Size: for stimulation of 1×109 cells