| Catalogue Number |
CYT-109 |
| Synonyms |
Glioma-derived growth factor, GDGF, Osteosarcoma-derived Growth Factor, ODGF, PDGF-AA, PDGF-1. |
| Introduction |
PDGF-AA, PDGF-BB and PDGF-AB, are potent mitogens for a variety of cell types including smooth muscle cells, connective tissue cells, bone and cartilage cells, and some blood cells. The PDGF is stored in platelet alpha-granules and released upon platelet activation. The PDGF is involved in a number of biological processes, including hyperplasia, chemotaxis, embryonic neuron development, and respiratory tubule epithelial cell development. Two distinct signaling receptors used by PDGF
have been identified and named PDGFR-alpha and PDGFR-beta. PDGFR-alpha is high-affinity receptor for each of the three PDGF forms. On the other hand, PDGFR-beta interacts with only PDGF-BB and PDGF-AB. |
| Description |
PDGF-AA Human Recombinant produced in HEK cells is a glycosylated homodimer, having a molecular weight range of 35-45kDa due to glycosylation.
The PDGF-AA is purified by proprietary chromatographic techniques. |
| Source |
HEK. |
| Physical Appearence |
Sterile Filtered White lyophilized (freeze-dried) powder. |
| Formulation |
The PDGF-AA was lyophilized from 1mg/ml in 1xPBS. |
| Solubility |
It is recommended to reconstitute the lyophilized PDGF-AA in sterile water not less than 100µg/ml, which can then be further diluted to other aqueous solutions. |
| Stability |
Lyophilized PDGF-AA although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution PDGF-AA should be stored at 4°C between 2-7 days and for future use below -18°C.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles. |
| Purity |
Greater than 95% as obsereved by SDS-PAGE. |
| Biological Activity |
The specific activity was determined by the dose-dependent stimulation of the proliferation of 3T3 cells and is typically 200-1000ng/ml. |
| Usage |
ProSpecs products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals. |
Наименование: Recombinant Human Platelet Derived Growth Factor-AA HEK / PDGF AA HEK.