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| / Каталог / Клеточные технологии и технологии для работы с клетками / Микроскопы. Микроскопическая техника / ELECTRON MICROSCOPY SCIENCES - Продукты для гистологии, световой микроскопии и электронной микроскопии / Chemicals for Microscopy | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Hydrochloric to Mucicarmine
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| RT | 16760 | 100 ml |
Hydrochloric Acid Solution 1.0N
HCl CAS #7647-01-0
Used to adjust the pH of buffers and fixative solutions.
| RT | 16770 | 100 ml | |
Hydrochloric Acid Solutions
For all our Hydrochloric Acid Solutions, please see listing inReagents for Histology.
HydrogenPeroxide 30% Solution-Stabilized, Reagent, A.C.S.
H2O2 F.W. 34.01 CAS #7722-84-1
| Assay | 29-32% | |
| Maximum of Impurities: | ||
| Chloride(Cl) | 0.0003% | |
| Nitrate | 0.0002% | |
| Phosphate | 0.0002% | |
| Sulfate | 0.0005% | |
| Heavy Metal | 0.0001% | |
| Iron | 0.00005% | |
| Titratable Acid | 0.0006meq/g |
| 0-5°C | 16790 | 100 ml | |
| 0-5°C | 16792 | 450 ml |
2-Hydroxyethyl Methacrylate
Please see (GMA) Glycol methacrylate.
| RT | 16800 | 450 ml |
HydroquinoneSolutions
For all our Hydroquinone Solutions, please see listing in Reagents for Histology.
(HPMA), 2-Hydroxypropyl Methacrylate
(Propylene glycol monomethacrylate)
C7H12O3 F.W. 144.17 CAS #27813-02-1
b.p. 70°C/1mmHg
Specific Gravity @25°C 1.028
Solubility in Water 13%
Inhibited with approx. 300ppm MEHQ
A water miscible monomer
| 0-5°C | 16900 | 450 ml | |
| 0-5°C | 16901 | 4x450 ml |
Imidazole, Reagent
(Glyoxaline)
C3H4N2 F.W. 68.08 CAS #288-32-4
Assay min 99.5%
Imidazole is a lipid-enhancing buffer agent. When used with 4% osmium tetroxide solution in the post-fixation of tissue blocks, it produces a prominent electron-opaque staining of lipid droplets with no evidence of diffusion.
Reference: Angermuller, S. Fahimi, H., Histochem. Journal 14, 823-35 (1982)
| RT | 16905 | 100g |
EMS Immersion Oils
EMS is proud to introduce a complete new line of immersion oils. This new family includes 5 unique types for
- Critical use
- Synthetic
- High viscosity
- Low viscosity with R.I 1.480 (Synthetic)
- Low refractive index (1.455) – suitable with aqueous media
A. EMS 255 Immersion Oil
Critical use – A synthetic immersion oil - for use with oil immersion lenses in microscopy. Manufactured and tested to a tight R.I. of 1.517 ± 0.001. The color is water-white to a light straw color. Comes complete with dispenser nozzle.
| RT | 16007-01 | EMS 255 | 30 ml |
| RT | 16007-02 | EMS 255 | 100 ml |
| RT | 16007-03 | EMS 255 | 10 L |
| RT | 16007-04 | EMS 255 | 25 L |
B. EMS 250 Immersion Oil
A medium viscosity oil formed from long chain hydrocarbons and polybutenes and terphenyls with a R.I of 1.518 ± 0.002 with water-white to a light straw color.
| RT | 16005-01 | EMS 250 | 30 ml |
| RT | 16005-02 | EMS 250 | 100 ml |
| RT | 16005-03 | EMS 250 | 10 L |
| RT | 16005-04 | EMS 250 | 25 L |
C. EMS 252 Immersion Oil
A high viscosity immersion oil to allow for work with slides mounted vertically to the lens, or in situations where slides are collected over a long period prior to being observed.
Manufactured from phthalate esters with a refractive index of 1.51 and is water-white to a light straw color.
| RT | 16007-05 | EMS 252 | 100 ml |
| RT | 16007-06 | EMS 252 | 5 L |
D. EMS 245 Immersion Oil
A low viscosity immersion oil which is suitable with aqueous media and has a R.I of 1.455.
| RT | 16006-01 | EMS 245 | 30 ml |
| RT | 16006-02 | EMS 245 | 100 ml |
| RT | 16006-03 | EMS 245 | 5 L |
E. EMS 265 Immersion Oil
A low viscosity immersion oil with a R.I of 1.480.
| RT | 16006-04 | EMS 265 | 30 ml |
| RT | 16006-05 | EMS 265 | 100 ml |
| RT | 16006-06 | EMS 265 | 10 L |
| RT | 16006-07 | EMS 265 | 25 L |
Immersion Oils For Normal Light Microscopy
Which Type - Which Application?
Cargille PCB-Free Immersion oils were introduced in 1972. Today, no other immersion oil meets FDA criteria. Now four standard types are available with the introduction of Type NVH, a very high viscosity oil. All Cargille Immersion Oils meet DIN 58 884 (Deutsche Institut Fur Normung e.V.)
For Normal Light Microscopy
Types A & B, with viscosities of 150 and 1250cSt respectively, meet these requirements. The greater the gap between cover glass and objective, or condenser and slide, the more desirable high viscosity becomes. For extremely large gaps, type NVH should be used. All Cargille Immersion Oils can be blended for intermediate viscosities.
37°C Immersion Oils
A number of research projects require microscopic studies at internal body temperature. Since the index/temperature relationship is inverse, increasing temperature significantly reduces the index of the immersion oil and consequently the imagery deteriorates. This problem has been resolved by the formation of Cargille 37°C Immersion Oils, which have an index of 1.515 at 37°C.
Consideration of these oils is also suggested where stage temperatures are elevated by sub-stage illuminators and high wattage projection equipment.
Immersion Oil Type 300
Automated Hematology Systems: Use Type 300; Automated Hematology Systems depend on accurate, precisely controlled physical and optical properties of immersion oil for successful imaging and mechanical processing. Type 300 is designed and manufactured to meet the stringent requirements of this equipment, which include specialized viscosity and exacting controls for its consistency.
Immersion Oils Specifications
OPTICAL & PHYSICAL PROPERTIES
| Immersion Oil Type | A | B | 300 | |
| Refractive Index : | @23°C | @23°C | @23°C | |
| F Line (4861 A) | 1.5239 | 1.5236 | 1.5238 | |
| E Line (5461 A) | 1.5180 | 1.5180 | 1.5180 | |
| D Line (5893 A) | 1.5150 | 1.5150 | 1.5150 | |
| C Line (6563 A) | 1.5114 | 1.5115 | 1.5116 | |
| Dispersion: | ||||
| nF-nC | 0.0125 | 0.0119 | 0.0123 | |
| Abbe VD | 41.3 | 43.2 | 41.8 | |
| Abbe Ve | 41.0 | 42.8 | 41.5 | |
| Temperature Coefficient (15-35°C) | -0.00033 | -0.00031 | -0.00033 | |
| Stability (change in nD25°C after 24 hrs @ temp.) | ||||
| at 60°C: | 0 | 0 | 0 | |
| at 100°C: | 0 | 0 | 0 | |
| Fluorescence (1): Short UV: | low | low | low | |
| Fluorescence (1): Long UV: | low | low | low | |
| Color: (Gardner) | 1 | 1 | 1 | |
| Viscosity: cSt +/- 10% @ 23°C (centistokes) | 150 low |
1250 high |
300 medium |
|
| Density: @ 23°C g/cc: | .923 | .923 | .923 | |
| Density: (US) lb/gal | 7.70 | 7.70 | 7.70 | |
| Cloud Point: | <-13°C | <-13°C | <-13°C | |
| Flash Point: | 325°F | 325°F | 325°F | |
| Neutralization No.: (mg KOH/g) | 0.01 max | 0.01 max | 0.01 max | |
(1) Relative to Cedarwood Oil
| RT | 16907-01 | TYPE A | |
1 oz |
| RT | 16907 | TYPE A | 4 oz | |
| RT | 16907-16 | TYPE A | 16 oz | |
| RT | 16908-01 | TYPE B | |
1 oz |
| RT | 16908 | TYPE B | 4 oz | |
| RT | 16908-16 | TYPE B | 16 oz | |
| RT | 16917 | TYPE 300 | |
1 oz |
| RT | 16917-01 | TYPE 300 | 4 oz | |
| RT | 16917-16 | TYPE 300 | 16 oz |
Special Immersion Oils
For Horizontal, Inverted and Inclined Instruments and Projection Equipment
Higher viscosities are generally required. Type NVH meets the requirement, having a viscosity of 21,000cST.
OPTICAL & PHYSICAL PROPERTIES
| Immersion Oil Type | 37** | NVH | OVH | |
| Refractive Index : | @37°C | @23°C | @23°C | |
| F Line (4861 A) | 1.5238 | 1.5230 | 1.5230 | |
| E Line (5461 A) | 1.5180 | 1.5178 | 1.5178 | |
| D Line (5893 A) | 1.5150 | 1.5150 | 1.5150 | |
| C Line (6563 A) | 1.5115 | 1.5118 | 1.5118 | |
| Dispersion: | ||||
| nF-nC | 0.0123 | 0.0113 | 0.0111 | |
| Abbe VD | 42.0 | 45.7 | 46.3 | |
| Abbe Ve | 41.7 | 45.4 | 46.0 | |
| Temperature Coefficient (15-35°C) | -0.00035 | -0.00034 | -0.00034 | |
| Stability (change in nD25°C after 24 hrs @ temp.) | ||||
| at 60°C: | 0 | 0 | 0 | |
| at 100°C: | 0 | 0 | 0 | |
| Fluorescence (1): Short UV: | low | low | low | |
| Fluorescence (1): Long UV: | low | low | low | |
| Color: (Gardner) | 1 | 1 | <3 | |
| Viscosity: cSt +/- 10% @ 23°C (centistokes) | 1250 high |
21,000 very high |
46,000 very high |
|
| Density: @ 23°C g/cc: | 0.917 | 0.919 | 0.918 | |
| Density: (US) lb/gal | 7.73 | 7.67 | 7.66 | |
| Cloud Point: | <-13°C | <-13°C | <-13°C | |
| Flash Point: | 325°F | 325°F | 325°F | |
| Neutralization No.: (mg KOH/gm) | 0.01 max | 0.01 max | 0.04 max | |
(1) Relative to Cedarwood Oil
**All values standardized at 37°C
| RT | 16914-01 | TYPE 37 | |
1 oz |
| RT | 16909-04 | TYPE 37 | 4 oz | |
| RT | 16909-01 | TYPE NVH | |
1 oz |
| RT | 16909 | TYPE NVH | 4 oz | |
| RT | 16918 | TYPE OVH | |
1 oz |
| RT | 16918-01 | TYPE OVH | 4 oz | |
| RT | 16918-16 | TYPE OVH | 16 oz |
Immersion Oils For Fluorescence Microscopy
After years of development and field testing, we now have available two new PCB-Free Immersion Oils for Fluorescence Microscopy.
* Immersion Oil Type HF
Type DF has been discontinued by Cargille. As an alternative we are offering either a new Immersion Oil called HF which would provide low fluorescence and the correct optical properties or you can use our FF which has virtually no fluorescence but slightly less than ideal optical properties.
* Immersion Oil Type FF
Type FF has virtually no background fluorescence which makes it ideal for observing low levels of fluorescence. It is highly stable, water white, and non-hydroscopic. Type FF can be substituted for all other fluorescence microscopy immersion oils in nearly all fluorescence applications.
* Immersion Oil Type LDF
Extremely low fluorescence is achieved by Type LDF. It is the replacement of choice for the DF which is no longer being manufactured. The LDF possesses the highest resolution of the oils for fluorescence applications.
OPTICAL & PHYSICAL PROPERTIES
| Immersion Oil | Type LDF | Type FF | Type HF | ||
| Refractive Index @ 23°C: | |||||
| F Line (4861 A) | 1.5239 | 1.4850 | 1.5234 | ||
| E Line (5461 A) | 1.5181 | 1.4811 | 1.5180 | ||
| D Line (5893 A) | 1.5150 | 1.4790 | 1.5150 | ||
| C Line (6563 A) | 1.5115 | 1.4766 | 1.5117 | ||
| Dispersion: | |||||
| nF-nC | 0.0125 | 0.0084 | 0.0118 | ||
| Abbe VD | 41.3 | 57.0 | 43.8 | ||
| Abbe Ve | 41.0 | 56.5 | 43.6 | ||
| Temperature Coefficient (15-35°C) |
-0.00036 | -0.00037 | -0.00038 | ||
| Stability (change in nD25°C after 24 hrs. @ temp.) | |||||
| at 60°C: | -0.0004 | 0 | +0.0001 | ||
| at 100°C: | -0.0043 | 0 | +0.0006 | ||
| Fluorescence (1): | |||||
| Short UV: | very low | none | verylow | ||
| Long UV: | very low | none | verylow | ||
| Color: (Gardner) | <1 | <1 | <1 | ||
| Viscosity: +/-10% @ 23°C (centistokes) | 500 medium |
170 low |
700 medium |
||
| Density: | |||||
| @ 23°C g/cc: | 0.984 | 0.877 | .9306 | ||
| (US) lb/gal | 8.21 | 7.32 | 7.77 | ||
| Cloud Point: | <-14°C | <-6°C | <2°C | ||
| Flash Point: | 171°F | 420°F | >340°F | ||
| Neutralization No.: (mg KOH/gm) | <0.01 max | 0.01max | 0.03max | ||
*Relative to Cedarwood Oil
| RT | 16919 | Immersion Oil Type LDF | |
1 oz |
| RT | 16919-01 | Immersion Oil Type LDF | 4 oz | |
| RT | 16919-16 | Immersion Oil Type LDF | 16 oz | |
| RT | 16915-01 | Immersion Oil Type HF | |
1 oz.* |
| RT | 16915-04 | Immersion Oil Type HF | 4 oz | |
| RT | 16916-01 | Immersion Oil Type FF | |
1 oz.* |
| RT | 16916-04 | Immersion Oil Type FF | 4 oz |
* 1 oz. Applicator bottles have a glass ball rod in the cap.
Immuno-Bed™ Embedding Kit
Please see listing in Embedding Media Kits.
ImmunoHistoZyme™, Ready-to-Use
Description: ImmunoHistoZyme is used for proteolytic digestion of formalin-fixed paraffin-embedded (FFPE) tissue sections prior to application of antibodies. In IHC most commonly used fixative like formalin mask tissue antigens (cellular, membrane and nuclear) by cross-linking process, this results in poor or no staining in IHC. ImmunoHistoZyme digestion of FFPE tissue section improves accessibility of antibodies to tissue antigens.
Storage: 2-8°C. DO NOT FREEZE
Intended Use:Use as antigen retriever for some antibodies in IHC. Please refer to primary antibody protocol for IHC.
Reagent: Ready-to-use ImmunoHistoZyme Reagent.
| 4-8°C | 64142-13 | ImmunoHistoZyme | 15 ml |
| 4-8°C | 64142-14 | ImmunoHistoZyme | 100 ml |
Indigo Carmine, Certified, C.N. #DcI-7
(Acid Blue 74; Carmine Blue; Indigotine 1, 1A)
C16H8N2O8S2Na2 F.W.466.36 CAS #860-22-0
Dye content: approx. 85%
Solubility: 1.3% Water; 0.0% Alcohol; 4.0% Cellosolve; 2.85% Glycol; 0.0% Xylene
Shunway's stain for animal embryos. Used with Acid Fuchsin for staining Negri bodies. Stain Tech., 5, 34 (1928)
Counterstain to Hematoxylin for staining vaginal smears. Am. J. Anat., 61, 505 (1937).
| RT | 16910 | 25g |
Technical Tip
Achieving Maximum Contrast with Stain.
Indium Trichloride, Anhydrous
InCl3 F.W. 221.18 CAS #10025-82-8
Chloride Content: 98%
A metal stain for nucleic acid
J. Biophys Biochem Cytol, 11,257 (1961)
| RT | 16920 | 5g |
Ingrain Blue, C.N. #AnAn-6
Please see Alcian Blue 8GX.
| RT | 10350 | 25g |
Isopropyl Alcohol, Reagent, A.C.S.
(2-Propanol)
CH3CH(OH)CH3 F.W. 60.10 CAS #67-63-0
A.C.S. Specifications:
| Color (APHA) | . | 10 |
| Density @ 25°C | . | 0.781-0.783 |
| Boiling Range (including 82.3°C) | . | 1°C |
| Titratable Acid or Base | . | 0.0001 meq/g |
| Residue after Evaporation | . | 0.001% |
| Solubility in water | . | To pass test |
| Water | . | 0.2% |
| RT | 16930 | 1qt | |
| RT | 16931 | 4x1qt |
JB-4(TM) Embedding Kit
Please see listing in Embedding Media Kits.
Karnovsky's Fixative
Please see Paraformaldehyde-Glutaraldehyde Solution.
Technical Tip
Elimination of Section Contamination During Staining
Kinyoun's Solution
Kinyoun's carbol fuchsin staining solution for acid-fast bacteria.
| RT | 26087-05 | Kinyoun's Solution | 500 ml |
| RT | 26087-06 | Kinyoun's Solution | 1 L |
Lanthanum Nitrate, Hexahydrate, Reagent
La(NO3)3·6H20 F.W. 433.03 CAS #10277-43-7
| m.p. | 40°C | |
| Assay | Min 99.9% | |
| Calcium | 0.001% | |
| Magnesium | 0.0001% |
A metal stain, intracellular tracer.
- Lane, N,J. and Treherne, J.E. (1970). Lanthanum staining of neurotubules in axons from cockroach ganglia. J. Cell Sci., 7:217
- Lane, N.J. (1972) Fine structure of a lipidopteran nervous system and its accessibility to peroxidase and lanthanum. Z. Zellforsch., 131:205
| RT | 17300 | 50g |
Lead Acetate, Trihydrate Reagent, A.C.S.
Pb(CH3COO)2·3H20 F.W. 379.33 CAS #6080-56-4
A.C.S. Specifications:
| Assay | . | 99.0-103.0% |
| Chloride | . | 5ppm |
| Copper | . | 0.002% |
| Insoluble Matter | . | 0.01% |
| Iron | . | 0.001% |
| Nitrate and Nitrite (as NO3) | . | To pass test |
| 0-5°C | 17600 | 100g | |
| 0-5°C | 17600-25 | 25g | |
*We are now offering Lead Acetate in 25 ml bottles to avoid its classification as a hazardous chemical for shipping purposes.
Lead Citrate, Trihydrate
Pb(C6H5O7)2·3H2O FW 1053.82 CAS #512-26-5
Minimum assay 99.%
The most widely used metal stain for ultra thin sections.
Reynolds, E.S., J. Cell Bio., 17, 208 (1963)
Sato, T., J. Electron Micro., 116, 133 (1976)
Venable, J.H. and Coggeshall, R. (1965). A simplified lead citrate stain for use in electron microscopy. J. Cell Biol., 25:407
Famy, A. (1967). An extemporaneous lead citrate for electron microscopy. Proc. 25th Ann. EMSA Meet., p.148. Claitor's Pub. Division, Baton Rouge, LA.
| RT | 17800 | 25g | |
| RT | 17810 | 100g |
Technical Tips
Handling of Lead Solutions
Lead Staining
Common Lead Stock Solutions
A Stable Lead Staining Solution
Lead Nitrate, Reagent, A.C.S.
Pb(NO3)2 F.W. 331.21 CAS #10099-74-8
| Minimum Assay | 99.5% | |
| Insoluble matter | 0.005% | |
| Reaction | Not less than pH 3.5 | |
| Chloride | 0.0005% | |
| Calcium | 0.01% | |
| Copper | 0.0005% | |
| Iron | 0.0005% | |
| Potassium | 0.002% | |
| Sodium | 0.002% |
A metal stain for ultra thin sections.
Sato, T., (1967) J. Electron Micro., 16, 733
| RT | 17900 | 25g |
Light Green SF Yellowish, Certified, C.N. #DcL-30
(Acid Green 5; Light Green 2G, S or 2GN; Acid Green F; Fast Acid Green N)
C37H34N2Na2O9S3 F.W. 792.86 CAS #5141-20-8
Solubility: 20.35% Water; 4.0% Alcohol; 5.25% Cellosolve; 12.0% Glycol; 0.0% Xylene
| RT | 17920 | 10 g |
Light Green SF Yellowish Solution
| RT | 26085-05 | Light Green SF Yellowish, 0.2% in 0.2% Acetic Acid |
500 ml |
| RT | 26085-06 | Light Green SF Yellowish, 0.2% in 0.2% Acetic Acid |
1 L |
| RT | 26085-10 | Light Green SF Yellowish, 1% Aqueous |
500 ml |
(SPURR), Low Viscosity, Embedding Kit
Please see listing in Embedding Media Kits.
| RT | 14300 | Low viscosity kit (Dr. Spurr) | |
kit |
Technical Tips
Embedding Resin Stain Permability
Lowicryl, Embedding Kit
Please see listing in Embedding Media Kits.
| RT | 14330 | Lowicryl K4M | |
kit |
| RT | 14340 | Lowicryl HM20 | |
kit |
| RT | 14350 | Lowicryl K11M | |
kit |
| RT | 14360 | Lowicryl HM23 | |
kit |
Technical Data Sheets and Newsletter
Lowicryl MonoStep Single Component Embedding Media
The same as our Lowicryl complete kits but this new formulation allows you one stop embedding without all of the cumbersome preparation and mixing. This pre-mixed, ready to use resin, saves you time and minimizes chemical contact. MonoStep Lowicryl is ideal for use in immunohistochemistry and immunolabeling. Store at room temperature.
| RT | 14335 | Lowicryl K4m Polar Embedding Medium; MonoStep |
|
225g |
| RT | 14345 | Lowicryl HM-20 Polar Embedding Medium; MonoStep |
|
225g |
LR White And LR Gold Resin
Please see listing in Embedding Media Kits.
Articles of Interest
Lucifer Yellow, CH, Dilithium Salt
C13H9Li2N2O9S2 F.W. 457.25 CAS #67769-47-5
A technique producing an electron dense osmiophilic polymer when Lucifer Yellow is injected into cells. Lucifer Yellow also can be viewed in living cells as a dye marker and is more sensitivethan any other dye.
Cell Tissue Res., 204, 37 (1979) J. Histochem Cystochem, 30, 189 (1982) Science, 217, 953 (1982); 215, 1641 (1982); 214, 801 (1981); 225, 851 (1984); Cell, 14, 741 (1978). A fluorescent dye. Nature 292, 17 (1981)
| 0-5°C | 17950 | 25mg |
Lugol's Iodine
For use as a bacterial, fungal and inclusion body stain.
| RT | 26055-05 | Lugol's Iodine Solution | 500 ml |
| RT | 26055-06 | Lugol's Iodine Solution | 1L |
Magnesium Chloride, Hexahydrate, Reagent, A.C.S.
MgCl2·6H2O F.W. 203.30 CAS #7791-18-6
Assay: 99.0-102.0%
| RT | 18010 | 100g |
Malachite Green 4, Certified C.N.#DcMg-11
Please see Basic Green Oxalate.
| RT | 18100 | 25g |
Maleic Acid, Crystalline, Repurified, A.C.S.
(Toxilic Acid; cis-Butenediodic Acid)
HOOCCH:CHCOOH F.W. 116.08 CAS #110-16-7
m.p. 132-134°C
Assay 99.5%
| Insoluble Matter | 0.005% | |
| Fumaric Acid | 0.15% | |
| Chloride | 0.01% | |
| Sulfate. | 0.001% |
| RT | 18150 | 250g |
Mammalian Ringer Solution
Ingredients: NaCl; KCl; CaCl2·6H2O; NaHCO3; MgCl2·6H2O; Glucose and Distilled water.
| RT | 11763-05 | Mammalian Ringer Solution | 500 ml |
| RT | 11763-10 | Mammalian Ringer Solution | 1 L |
May-Grunwald Staining Solution
A ready-to-use stain for bone marrow staining.
| RT | 26064-05 | May-Grunwald Stain Solution | 500 ml |
| RT | 26064-06 | May-Grunwald Stain Solution | 1 L |
Mayer's Hematoxylin
Stain selectivity for nuclei. Ready-to-use.
| RT | 26043-05 | Mayer's Hematoxylin | 500 ml |
| RT | 26043-06 | Mayer's Hematoxylin | 1 L |
| RT | 26043-10 | Mayer's Hematoxylin | 4 L |
Modified Mayer's Hematoxylin
Stock Solution. Ready-to-use.
Ingredients: Hematoxylin 0.4%, Ammonium Aluminum Sulfate 0.5%,
Sodium Iodate: 0.03%, Citric Acid 0.1%, Chloral Hydrate 5%
| RT | 26043-18 | Modified Mayer's Hematoxylin | 500 ml |
Methacrylic Acid, Glacial
(2-Methylpropenoic Acid)
CH2:C(CH3)COOH F.W. 86.09 CAS #79-41-4
Assay: >98%
Stab. with 0.25% Hydroquinone monomethyl ether
| RT | 18500 | 450 ml |
Methanol, Reagent, A.C.S.
(Methyl Alcohol)
CH3OH F.W. 32.04 CAS #67-56-1
Assay: 99.8%
Specific Gravity : 0.79
A.C.S. Specifications:
| Residue after Evaporation | . | 0.001% |
| Boiling Range (Boiling point 64.6°C) | . | 2°C max |
| Appearance | . | Clear |
| Color (A.P.H.A.) | . | 10 max |
| Acetone & Aldehydes (0.001%) | . | To pass test |
| Titratable Acid | . | 0.0003 meq/g |
| Titratable Base | . | 0.0002 meq/g |
| Water | . | 0.2% max |
| RT | 18510 | 1qt | |
| RT | 18511 | 4x1qt |
Methenamine
Please see Hexamethylenetetramine.
| RT | 16710 | 50g |
Methenamine Solution
A stock solution for use in bacterial and fungal stains (Grocott's Method/GMS).
| RT | 26083-05 | Methenamine, 3% Aqueous Solution | 500 ml |
| RT | 26083-06 | Methenamine, 3% Aqueous Solution | 1 L |
2-Methyl Butane, Reagent
(Isopropentane)
CH2H5CH(CH3)2 F.W. 72.15 CAS #78-78-4
b.p. 27-31°C
Specific Gravity @ 20°C: 0.62
| RT | 18550 | 450 ml |
Methyl Cellulose, 400 cPS,USP/NF
(Methylcel MC; Cellulose Methyl Ether)
CAS #9004-67-5
Methoxyl (-CH3) content : 27.5-31.5%
Medium viscosity (2% in water, @20°C): 230-550 cps
Used with polyethylene glycol in the presence of acidic uranyl acetate to stain cryo sections.
| RT | 18560 | 100g |
MethyleneBlue Chloride, Certified, C.N. #DcA-24
(Basic Blue 9; Swiss Blue)
C16H18N3SCl •3H2O F.W. 319.86+aq CAS #7220-79-3
Solubility: 9.5% Water; 6.0% Alcohol; 6.5% Cellosolve; 10.0% Glycol; 0.0% Xylene.
- Used to distinguish autonomic nerves. Richardson, K.C., (1969) Anat. Rec., 164, 231. General stain for animals, histology.
- A simple rapid staining procedure for methacrylate embedded tissue sections using chromotrope 2R and Methylene Blue. Dougherty, M.M., and King, J.S., (1984) Stain Tech., 59, 149
- An established routine method for differential staining of epoxy-embedded tissue sections. Schroeder, H.E., Rossinsky, K., and Muller, W., (1980) Microsc. Acta 83, 111
- Used with Basic Fuchsin for stain epoxy-embedded tissue. Sato, T. and Shamoto, M. (1973). A simple rapid polychrome stain for epoxy-embedded tissue. Stain Technol., 48:223
| RT | 18600 | 25g |
Technical Tips
A Methylene Blue-Azure II Stain
Methylene Blue Solutions
Please see listing in Prepared Staining Solutions
Methyl Ethyl Ketone, Reagent
(2-Butanone)
CH3COCH2CH3 F.W. 72.11 CAS #78-93-3
| Assay | >98% | |
| Specific Gravity (H2O=1) : | 0.799-0.802 | |
| Acidity (as CH3COOH) | 0.003% | |
| Water | 0.2% |
| RT | 18650 | 450 ml |
Methylene Chloride, Reagent, A.C.S.
(Dichloromethane; Plastisolve)
CH2Cl2 F.W. 84.93 CAS #75-09-2
| RT | 19600 | 1qt | |
| RT | 19610 | 4x1qt |
Technical Tips
Methyl Green, Certified, C.N. #DcG-10
(Zinc Chloride Salt; Ethyl Green)
C27H35N3C12·ZnC12 F.W. 608.79 CAS #7114-03-6
Solubility: 8.0% water; 3.0% Alcohol; 1.8% Cellosolve; 3.5% Glycol; 0.0% Xylene.
- Used as a counterstain in Almann's aniline acid fuchsin technique for mitochrondria; used in Kurnick's Methyl Green-Pyonin stain for nuclei and cystoplasmic granules of liver cells. Marker for DNA at acid and neutral conditions. J.B. Boyd, H.R. Mitchell, (1965) Anal. Biochem., 13, 28.
- Used for epoxy-embedded thick sections. Sievers, J. (1971).
- Basic two-dye stains for epoxy-embedded 0.3-1 micron sections. Stain Technol., 46:195
| RT | 18700 | 25g |
Methyl Methacrylate, Monomer
(Methacrylic Acid Methyl Ester)
CH2·C(CH3)COOCH3 F.W. 100.12 CAS #80-62-6
b.p. 99-100°C
Specific Gravity @20°C : 0.943
Inhibitor (Hydroquinone): 0.0025% (25ppm)
| 0-5°C | 18800 | 225 ml |
Methyl Methacrylate/Butyl Methacrylate Embedding Kit,
Please see listing in Embedding Media Kits
Methyl Violet 2B, Certified, C.N. #DcMv-5
(Basic Violet 1; Dahlia B; Gentian Violet; Paris Violet; Pyoktanium Coeruleum)
C24H28N4Cl F.W. 393.96 CAS #8004-87-3
Solubility: 9.0% Water; 8.25% Alcohol; 9.0% Cellosolve; 8.5% Glycol;0.01% Xylene
Dye content: 75% min
Used with Methyl Green to stain Epoxy-embedded sections.
| RT | 18850 | 25g |
Michel's Fixative
Please see listing in Reagents for Histology
Microbed Embedding Kit
Please see listing in Embedding Media Kits
Millonig's Phosphate Buffer
Please see listing in Prepared Buffers
| 0-5°C | 11582-05 | 500 ml | |
| 0-5°C | 11582-10 | 1 lit |
Molybdic Acid, Ammonium Salt
Please see ammonium molybdate
| RT | 10500 | 100g |
MOPS, Biological Buffer
(3-(N-Morpholino) propanesulfonic acid)
C7H15NO4H F.W.209.26 CAS #1132-61-2
Assay: >99.%
| RT | 18900 | 100g |
Mucicarmine/Metanil
For use with hematoxylin and metanil yellow for the determination of carbohydrates and mucosubstances.
| RT | 26080-05 | Mucicarmine Stock Solution | 500 ml |
| RT | 26080-06 | Mucicarmine Stock Solution | 1 L |
| RT | 26081-05 | Metanil, 0.25% Stock Solution | 500 ml |
| RT | 26081-06 | Metanil, 0.25% Stock Solution | 1 L |
Информация для заказа
| Наименование | Объем | Производство | Метод | Кат.Номер |
|---|---|---|---|---|
| Цитрат свинца, 100 г | 100g | Electron Microscopy Sciences | EMS17810  |
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