Glycerine to HPMA

Fluorescein Isothiocyanate, Isomer I, Certified (FITC)

C₂₁H₁₁NO₅S   F.W. 389.39   CAS #3326-32-7
Assay >90%
Water soluble.

Fluoromount-G™, Slide Mountant

Please see listing in Adhesives and Mountants.

Formamide, Ultra High Purity

HCONH₂   F.W. 45.04   CAS #75-12-7
Specific Gravity: 1.128-1.132
Glass Distilled. Exceeds A.C.S. Specifications
Specifications:

Formic Acid 96%, Reagent, A.C.S.

HCOOH   F.W. 46.03   CAS #64-18-6
Specific Gravity: 1.22

Formvar 15/ 95 Resin, Powder

CAS #63450-15-7
Polyvinyl formvar desiccated. Used to prepare Support films.
Soluble in ethylene dichloride, chloroform or dioxane.

Formvar Solution in Ethylene Dichloride

Microfiltered-ready to use.

RT	15810	0.25%		100 ml
RT	15820	0.50%		100 ml
RT	15830	1.00%		100 ml
RT	15830-25	1.00%		4x25 ml

Gach Kit

Please see listing in Embedding Media Kits.

RT	15920	Kit consists of:		kit
		10x10 ml 50% EM grade Glutaraldehyde
		10x1.5g Carbohydrazide

Technical Tip:

Preparation of Gelatin Adhesive

Gelatin, Powder, U.S.P.

CAS #9000-70-8
Solubility: 12% in H₂O at 20°C
pH (1% in H₂O): 3.5-5.0
Residue after ignition: <2%
Type A-from Porcine skin (acid cured tissue)
Type B-from Bovine skin (base cured tissue)
The strength of the gel is based on the bloom number. The higher the bloom number the stronger the gel.

• Used as a combination Acrylamide-Gelatin-Jung resin for cryosectioning of large and soft tissues (eggs, fishes, insects) Hartmann, R. (1984). A new embedding medium for cryosectioning eggs of high yolk and lipid content. Eur. J. Cell Biol., 34:206
• Used to prepare gelatin-chrome alum adhesive to coat glass slides for mounting semi-thin sections for staining.

RT	16560	Gelatin, Type A; × 60 Bloom		100g
RT	16562	Gelatin, Type A; × 175 Bloom		100g
RT	16564	Gelatin, Type A; × 300 Bloom		100g
RT	16566	Gelatin, Type B; × 75 Bloom		100g
RT	16568	Gelatin, Type B; × 225 Bloom		100g

Giemsa Solution, C.N.#DcGe-19

Specific Gravity: 0.89
Differentiates types of leucocytes, bacteria and inclusion bodies.
Giemsa, Centralbl. F. Publ., Abt. 1,37, 308 (1904) Stain for thick blood films; shows malarial parasites. Barber and Komp, Publ. Health Rep., 44, 2330 (1929) Bone marrow and stain for showing cell morphology; together with May-Grunwald Solution for pap staining. Pappenheim, Folia Haemat., 13, 338 (1912)

Giemsa Stain, C.N.#DcGe-19, Powder

CAS #51811-82-6
Please see listing in Certified Dyes/Stains.

For our Giesma Stain Solution

Please see listing in Histology: Certified Dyes/Stains.

For our Differential Quick Staining Kit (Modified Giesma) Stain Solution

Please see listing in Histology: Prepared Staining Solutions.

Gill's Hematoxylin Staining Solution

Gill's Hematoxylin is a specially formulated solution for use as a biological stain for the nuclei and nuclear chromatin of normal and abnormal cells whether in whole or sliced cells. Our Gill's Hematoxylin is designed to use at its full strength. It is microfiltered prior to shipment; therefore, filtration before use is not required. For repeated use further filtration is recommended. At the discretion of the user, the Gill's Hematoxylin No. 2 and No. 3 may be diluted with ethylene glycol (25% is recommended) to produce a weaker stain solution.

RT	26030-10	Gill's Hematoxylin No. 1		1 L
RT	26030-14	Gill's Hematoxylin No. 1		4 L
RT	26030-20	Gill's Hematoxylin No. 2		1 L
RT	26030-24	Gill's Hematoxylin No. 2		4 L
RT	26030-30	Gill's Hematoxylin No. 3		1 L
RT	26030-34	Gill's Hematoxylin No. 3		4 L

Glycerine, Reagent, A.C.S.

(Glycerol)
C₃H₅(OH)₃   F.W. 92.10   CAS #56-81-5

(GMA), Glycol-Methacrylate, Low Acid

(2-Hydroxyethyl methacrylate monomer, inhibited;HEMA)
C₆H₁₀O₃   F.W. 130.14   CAS#868-77-9
Specific Gravity: 1.07
b.p. 67°C
Inhibited with approximately 330ppm MEHQ
A water soluble monomer.

Article of Interest

Preparation of Glycol Methacrylate

Glyoxal 3% Fixative, Ready to Use

An alternative fixative to Formalin and Formaldehyde.

EMS is proud to introduce an alternative to Formalin and Formaldehyde in many different applications.

Our unique kit comes premixed and measured and ready to use. The kit consists of 2 vials: Solution A & Solution B (Absolute Ethanol, Glyoxal Solution, Acetic Acid and water).

Instructions:

Add solution A to solution B. Vortex the two solutions together and bring to a pH of 4.5 with the addition of NaOH 1M. The solution needs to be used immediately once mixed.

References

Sabatini DD. et al (1963) "The preservation of cellular ultrastructure and enzymatic activity by aldehyde fixation". J. Cell Biology. 17:19-58

Janigan DT. et al (1965) "The effects of aldehyde fixation on acid phosphatase activity in tissue blocks". J. Histochemistry. Cytochemistry. 13: 476-483

Dapson RW. et al (2006) "Glyoxal fixation and its relationship to immunocytochemistry." J. Histotechnology. 29: 65-76

Dapson RW. Et al (2007) "Glyoxal fixation: how it works and why it only occasionally needs antigen retrieval" lnforma healthcare, Dapson & Dapson, LLC

Richter K. et al (2018) "Glyoxal as an alternative to formaldehyde in immunostaining and super-resolution microscopy" The EMBO Journal. 37: 139-159

GMA Embedding Kit

Please see listing in Embedding Media Kits.

RT	14200	Kit consists of:		kit
		450 ml Glycol methacrylate, GMA
		225 ml n-Butyl methacrylate
		15 gm Benzoyl Peroxide Paste

GMA-PEG Embedding Kit Polyethylene Glycol- Glycol methacrylate mixture.

Please see listing in Embedding Media Kits.

RT	14250	Kit consists of:		kit
		450 ml Glycol methacrylate, GMA
		225 ml n-Butyl methacrylate
		50 ml Ethylene glycol dimethacrylate
		20 ml Polyethylene Glycol 400
		15g Benzoyl Peroxide Paste

Gold Chloride, Hydrate(Chloroauric Acid; Hydrogen Tetrachloroaurate)

H(AuCl₄)·H₂O   F.W. 357.79   CAS #16961-25-4
Assay 99.9985%
Gold contents 49%+
A highly purified gold compound for the preparation of Colloidal Gold sols. Preweighed to facilitate your work. (Preparation instructions for Gold Sols included)

Gold Chloride Solutions

Please see listing in Reagents for Histology.

Tannic Acid, used to prepare gold sols

Please see listing in Chemicals.

Gram's Iodine Solution

For use as a bacterial, fungal and inclusion body stain.

Graphite Adhesives

Please see listing in Colloidal Compounds.

Gum Mastic

Please see listing in Reagents for Histology.

HANKS's Balanced Salt Solution (HBSS)

All of our Hanks' Balanced Salt Solutions contain: Potassium Chloride, Potassium Phosphate Monobasic, Sodium Chloride, Sodium Phosphate Dibasic, and D-Glucose. Some formulations may contain additional components. Use the table below as a quick reference for identifying the one that best suits your application. All solutions are prepared in 1X strength.

RT	11768-05	HBSS, Formula I, 0.179M		500 ml
RT	11768-10	HBSS, Formula I, 0.179M		1 L
RT	11769-05	HBSS, Formula II, 0.155M		500 ml
RT	11769-10	HBSS, Formula II, 0.155M		1 L
RT	11770-05	HBSS, Formula III, 0.147M		500 ml
RT	11770-10	HBSS, Formula III, 0.147M		1 L

Harris' Hematoxylin

Stain selectivity for nuclei, fat and lipids. Ready-to-use.

RT	26041-05	Harris' Hematoxylin		500 ml
RT	26041-06	Harris' Hematoxylin		1 L
RT	26041-20	Harris' Hematoxylin without Mercury		500 ml

Hematoxylin, Certified, C.N.#DcH-48

(Natural Black I, Hydroxybrasilin)
C₁₆H₁₄O₆   F.W. 302.29   CAS #517-28-2
Solubility: 10.0% Water; 10.0% Alcohol; 9.5% Cellosolve; 10% Glycol; 0.0% Xylene. A certified biological stain used in histology and cytology and plastic embedded tissues.
Chen, Chang, S., J Arch. of Path., 93,334 (1972).

Technical Tip

Hematoxylin Staining Tips

Hepes 0.2M Buffer Solution

Please see listing in Prepared Buffers.

Hepes, Crystal, Biological Buffer

(N-2-Hydroxyethylpiperazine-N'-2-Ethanesulfonic acid)
C₈H₁₈O₄N₂S   F.W. 238.30   CAS #7365-45-9

(HMDS), Hexamethyldisilazane, Reagent

(CH₃)₃SiNHSi(CH₃)₃   F.W. 161.40   CAS #999-97-3
b.p. 126.7°C
Assay >97.0%
Specific Gravity @25°C 0.774
Refractive Index @20°C: 1.407
* For fast preparation of soft insect tissues for SEM. A good substitution for critical point drying. Int. J. Insect Morh. Embryol., 12(4), 201 (1983) Stain Tech., 58(6), 347 (1983)
* Bray, D.F., Et-al. (1993). Micros. Res. and Technique. 26:489-495. A comparison of HMDS, Peldri II, and critical point drying methods for SEM of biological specimens.

Hexamethylenetetramine A.C.S. Reagent, Granular

(Urotropine; Methenamine)
C₆N₄   F.W. 140.19   CAS #100-97-0
Assay: 99.0-100.5%
Specifications:

Used in silver stains.
Swift, J.A. (1968) Microsc. Soc. 88, 449.

*We are now offering Hexamethylenetetramine in 25 ml bottles to avoid its classification as a hazardous chemical for shipping purposes.

Hexylene Glycol, Reagent, A.C.S.

(2-Methyl-2,4-pentanediol)
(CH₃)₂C(OH)CH₂CH(OH)CH₃   F.W. 118.18   CAS #107-41-5
Specific Gravity @25°C 0.923
A.C.S. Specifications:

Mixed with water in all proportions. Used as a dehydrating agent for fat tissues.

HPMA Embedding Kit

Please see listing in Embedding Media Kits.

HRP Conjugate Diluent

Specifically designed for preparing solution of your HRP-conjugate used as the detection reagent. It is the Antibody-dilutent buffer with additional component for stabilizing your HRP-conjugate. Allows you to further standardize the assay preparing ready-to-use conjugate solutions in advance and store them in refrigerator without loss of activity.

Наименование	Объем	Производство	Метод	Кат.Номер
Formvar Solution in Ethylene Dichloride 0.25%, 100 мл		Electron Microscopy Sciences		15810