Safranin to Sym-Collidine

Safranin to Sym-Collidine

Safranin O, Certified, C.N. #DcS-25

(Safranin T, Y or A; Basis Red 2; Cotton Red; Gossypimine
C₂₀H₁₉N₄Cl   F.W. 350.85   CAS #477-73-6
Solubility: 4.5% Water; 3.5% Alcohol; 5.0% Cellosolve; 3.5% Glycol; 0.0% Xylene

Schiff's Reagent

For use in the Periodic Acid Leucofuchsin (PAS) method as a general tissue stain.

Scott's Tap Water Substitute

Magnesium sulfate buffered with sodium bicarbonate. Ready-to-use.

Section Block

A new class of blocking solutions based on chemically modified and fragmented ultra-pure casein. Effectively reduces unwanted binding of primary antibody and conjugates you use to charged surface of the slide and tissue section. Greatly reduces non-specific binding while preserving the specific reaction, by saturating potential non-specific protein-protein interactions. Moreover, in contrast to BSA-based, IgGm casein or serum-based blocking solutions there is no interaction of specific antibody and blocking protein itself.It is not comparable to other commercially available or home-made blocking solutions. Recommended for research and diagnostic pathology, especially for retrieved sections and polyclonal antibodies.

Silver, Collodial

Please see our complete listing.

Silver Lactate, Powder

CH₃CH(OH)CO₂Ag   F.W. 196.94  CAS #128-00-7
Silver content: approx. 90% min.
* To localize intracellular anions (Cl-). Komnick, (1962).
* Elektronemikrokopische Lokalisation von Na+ und Cl in Zellan und Geweban. Protoplasma, 55,414.

Silver Protein, Strong

Protargol, 8.3% Ag  CAS #9008-42-8
Thiery, (1967). See Silver Nitrate

Silver Nitrate, Reagent, A.C.S.

AgNO₃   F.W. 169.89  CAS #7761-88-8
Assay 99.9+%
A.C.S. Specifications:

References for Silver stain:

• Swift, J.A. (1968). The electron histochemistry of cystine-containing proteins in thin transverse sections of human hair. J.R. Microsc. Soc. 88,449.
• Thiery, J.P. (1967). Mise en evidence des Polysaccharides sur coupes fines en microscopie eletronique. J. Microsc. (Paris) 6,987.
• Rambourg, A. (1967). An improved silver methenamine technique for the detection of periodic acid-reactive complex carbohydrates with the electron microscope.J. Histochem. Cytochem. 15, 409.
• Hermandez et al., (1968)). Periodic acid-chromic acid-methenamine silver technique for glycoprotein detection in the electron microscope. J. Histochem. Cystochem. 16, 507.
• Ribi, W.A. (1976). A golgi-electron microscope method for insect nervous tissue. Stain Technol. 51,13.
• Braak, H., and Braak, E. (1982). A simple procedure for electron microscopy of Golgi-impregnated nerve cells. Neurosci. Lett. 32,1.

Silver Nitrate Solutions

Please see listing in Reagents for Histology.

(S-TPPS) Silver Tetraphenylporphin Sulfonate

An EM stain for elastin. Albert, E.N., And Fleischer E. (1970).
A new electron dense stain for elastic tissue. J. Histochem.Cystochem. 18, 697

Sodium Acetate, Trihydrate Reagent, A.C.S.

CH₃COONa·3H₂O   F.W. 136.08   CAS #6131-90-4
A.C.S. Specifications:

 Sodium Bicarbonate, Powder, Reagent, A.C.S.

NaHCO₃   F.W. 84.01   CAS #144-55-8
Assay 97.7-100.3%
Specifications:

Sodium Bisulfite Solution

A 1% aqueous stock solution for fungal staining.

Sodium Borate, Tetra, Granular, Reagent, A.C.S

Please see Borax Tetra.

Sodium Cacodylate Trihydrate Sodium dimethyl arsenate,

Please see Cacodylic Acid, Sodium Salt.

Sodium Cacodylate, Buffer Solution

Please see listing in Prepared Buffers.

Sodium Carbonate, Anhydrous, Reagent, A.C.S.

Na₂CO₃   F.W. 105.99   CAS #497-19-8
Specifications:

Sodium Citrate, Granular, Reagent

Na₃C₆H₅O₇·2H₂O   F.W. 294.11   CAS #6132-04-3

Sodium Ethylate

(Sodium Ethoxide)
C₂H₅ONa   F.W. 68.05   CAS #141-52-6
Used as an agent for flattening thick sections (0.5-2.0 micron) of epoxy embedded material. Felix Sierra, Christa Hegele-Hartung and Henning M. Beier (1990).
An improved technique for flattening semithin sections of epoxy-embedded tissues used for high resolution light microscopy. J. of Microscopy Vol 159, Pt 1, (July 1990), pp. 95-98

Technical Tips

An Improved Technique for Flattening Semithin Sections of Epoxy-Embedded Tissues Used for High-Resolution Light Microscopy

Sodium Hydroxide, Pellets, Reagent, A.C.S.

NaOH   F.W.40.00   CAS #1310-73-2
Assay >97.0%
Specifications:

Sodium Hydroxide, Solution

2N Standardized   CAS #1310-73-2
Carbonate-free. This solution is prepared from ACS Reagent grade sodium hydroxide, and is standardized colormetrically against potassium biphthalate primary reference material.
Normal Concentration 1.98-2.02

Sodium Methoxide, Anhydrous

(Sodium Methylate)
CH₃ONa   F.W. 54.03   CAS #124-41-4
Used in the removal of resin from sections.

Sodium Phosphate, Dibasic, Heptahydrate, Reagent, A.C.S.

Na₂HPO₄·7H₂O   F.W. 268.07   CAS #7782-85-6

Sodium Phosphate, Monobasic,

Hydrate,Reagent, A.C.S.
NaH₂PO₄·H₂O   F.W. 137.99   CAS #10049-21-5
Assay 98.0-102%
pH of a 5% solution @25°C-4.1-4.5
Specifications:

Technical Tips

Temperature Conversions

Sodium Sulfite, Reagent, A.C.S.

Na₂SO₃   F.W. 126.04   CAS #7757-83-7
Assay >98.0%
Specifications:

Sodium Tartrate, Reagent, A.C.S.

Na₂C₄H₄O₆·2H₂O   F.W. 230.08   CAS #6106-24-7
Assay 99.5%-101.0%
pH of a 5% solution @25°C-7.0-9.0
Loss on Drying @150°C-15.61-15.71%

Specifications:

Sodium Tungstate, Reagent, A.C.S.

Na₂WO₄.2H₂O   FW 329.86   CAS #10213-10-2
Assay 99.0-101.0%
Specifications:

• Stockert,J.C. (1977). Sodium Tungstate as a stain in electron microscopy. Biol. Cellul., 29:211
• Takeuchi, I.K. (1981). Differential staining of nucleoli and chromatin by sodium tungstate. J. Electron Microsc., 30:15

Sorensen's Phosphate Buffer

Please see listing in Prepared Buffers.

(SPURR), Low Viscosity Embedding Kit

Please see listing in Embedding Media Kits.

Staining Solutions

We now offer a complete line of prepared, ready-to-use, high quality staining solutions for all standard staining procedures used by the Biological Staining Commission and the Armed Forces Institute of Pathology.

• All solutions and concentrations are packaged and prepared in convenient and precise quantities
• Premixed ready-to-use; saves you time and effort
• Consistently uniform results are obtained.

For a complete list and description of all of our stains, please see Chemicals for Microscopy

For Prepared Staining Kits, please see the complete listing of Prepared Staining Solutions

Technical Tips

Improving the Adhesion of Sections Filmless Grids

Styrene, Reagent

(Vinylbenzene)
C₆H₅CH:CH₂   F.W. 104.14   CAS #100-42-5
Specific Gravity (H₂O=1): 0.909
Assay >99.0%
Stabilizer (p-tert-Butylcatechol)-10-15ppm
A monomeric styrene used as an embedding media for ultramicrotomy.

• DeLamater, E.D., et al (1971). The use of styrenes as embedding media for electron microscopy. Pro. 29th Ann. Meet. EMSA, p. 488. Claitor's Pub. Divsion, Baton Rouge, LA
• Kushida, H. (1962b). On ultraviolet polymerization of styrene resins in embedding for electron microscopy. J. Electron Microsc., Vol. 2. p.10. Academic Press, New York.
• Kushida, H. (1961a). A styrene-methacrylate resin embedding method for ultrathin sectioning. J. Electron Micro., 10:16

Styrene Embedding Kit

Please see listing in Embedding Media Kits.

Succinic Acid, Crystal, Reagent, A.C.S.

(CH₃COOH)₂   F.W. 118.09   CAS #110-15-6
m.p. 185-191°C
Assay >99.0%
Specifications:

Sucrose, Reagent, A.C.S.

C₁₂H₂₂O₁₁   F.W. 342.20   CAS #57-50-1
Specifications:

Sudan Black B, Certified, C.N. #DcZb-2

(Solvent Black 3)
C₂₉H₂₄N₆   M.W. 456.56   CAS #4197-25-5
Solubility: Water-insoluble; Alcohol-soluble; 4.0% Cellosolve; 1.0% Glycol.

Sure-cut Surfactant

Epoxy resins are easier to section with glass, as well as diamond knives, when the embedding medium contains 0.5-1.0% of our SURE-CUT. The life of the diamond knife will be extended with its use because it will reduce the friction of the plastic block during sectioning.

Sym-Collidine, Buffer kit

Please see listing in Prepared Buffers.

Sym-Collidine, Reagent

Please see listing in Prepared Buffers.

Наименование	Объем	Производство	Метод	Кат.Номер
Какодилат натрия, буферный раствор	500 мл	Electron Microscopy Sciences		EMS 11652